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The in Vitro Effect of Oxidized LDL and PHA on Proliferation and Gene Expression of Regulatory T Cells in Patients with Atherosclerosis

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Author(s): Azadeh Mottaghi | Eisa Salehi | Hashem Sezavar | Seyed Ali Keshavarz | Mohammad Reza Eshraghian | Nima Rezaei | Leili Rejali | Ali-Akbar Saboor-Yaraghi

Journal: Iranian Journal Of Allergy, Asthma and Immunology
ISSN 1735-1502

Volume: 11;
Issue: 3;
Start page: 217;
Date: 2012;
Original page

Keywords: Atherosclerosis | Gene Expression | Ox-LDL | Peripheral Blood Mononuclear Cells | T Regulatory Cells

ABSTRACT
Atherosclerosis is a chronic inflammatory condition that affects the arterial wall. Oxidized low-density lipoprotein (ox-LDL) seems to have an important role in atherosclerotic plaque formation.This study was performed to investigate the effects of ox-LDL as well as PHA on proliferation and gene expression of peripheral blood mononuclear cells (PBMCs) in patients with atherosclerosis compared to healthy controls. Proliferation of PBMCs was assessed by BrdU assay, while gene expression was assessed by real-time PCR.Both PHA and ox-LDL significantly induced proliferation of PBMCs of patients and controls. PBMCs from controls showed significantly higher proliferation when stimulated with ox-LDL compared to patients. Expression of TGF-β was significantly lower in PBMCs from patients compared to healthy controls (p
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