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In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation

Author(s): Cenap Ekinci | Aysun Ekinci | Bülent Ahıshalı | Ayhan Bilir

Journal: Journal of Clinical and Experimental Investigations
ISSN 1309-8578

Volume: 3;
Issue: 3;
Start page: 350;
Date: 2012;
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Keywords: Ellagic acid | C6 glioma cells | cell culture | brain tumor | glioma

Objectives: Ellagic acid is a plant-derived polyphenoliccompound. The aim of this study is to investigate in vitroeffects of ellagic acid on the proliferation and viability ofrat C6 glioma cell lineage depending on dose and timeand also is to evaluate ultrastructural changes in C6 gliomaspheroids.Materials and methods: Effects of ellagic acid on rat C6glioma cell line were investigated by using two-dimensionalmodels of tumor cell culture depending on doseand time. The effects of Ellagic acid was evaluated ascell proliferation, cell viability, and synthesis phase of cellcycle, and cell structure at 24th, 48th, 72nd hours. Cell structurewas evaluated at 24th and 72nd hours in three dimensionalcell culture spheroid models.Results: Reducing effect of ellagic acid on cell proliferationand cell viability was seen in two dimensional cultures(p0. 05). Electronmicrographs in which three dimensional cell culturespheroid models was investigated, structural changeswas found to be different from the control group.Conclusions: In the evaluation of effects of ellagic acidon rat C6 glioma cells using two-dimensional culture models,it was observed that ellagic acid caused reduced cellviability, deterioration in cell structure and prevented cellproliferation. J Clin Exp Invest 2012; 3 (3): 350-356Key words: Ellagic acid, C6 glioma cells, cell culture,brain tumor, glioma
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