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In vitro evaluation of polihexanide: biocompatible and effective

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Author(s): Wiegand, Cornelia | Abel, Martin | Ruth, Peter | Hipler, Uta-Christina

Journal: GMS Krankenhaushygiene Interdisziplinär
ISSN 1863-5245

Volume: 4;
Issue: 2;
Start page: Doc15;
Date: 2009;
Original page

Keywords: HaCaT keratinocytes | polihexanide | Staphylococcus aureus | wound infection

ABSTRACT
Infection may lead to the formation of a chronic wound or is a common complication during their treatment. Rather than relying on just debriding and cleansing the wound, additional therapeutic strategies are commonly applied in an attempt to prevent infection. Therefore, wound dressings combined with antimicrobial agents such as silver, povidine iodine, or polihexanide are increasingly utilized in the treatment of critical colonized or infected chronic wounds. Polihexanide is regarded first choice as therapy option because of its good skin tolerance beside its antimicrobial effects. Furthermore, a positive influence of polihexanide on wound closure was observed in a study with aseptic wounds in piglets. Moreover, polihexanide is able to induce cell proliferation in vitro. In vitro test systems provide valuable tools in the study of substance or material effects on cells. They use highly defined culture conditions and avoid the complex mechanisms which occur in vivo and thus allow the direct measurement of the influence on cell viability and proliferation. For instance, the anti-oxidative effect can be determined and the antimicrobial activity measured in vitro. Furthermore, a co-culture system of HaCaT keratinocytes and Staphylococcus aureus was used to test the capacity of polihexanide to protect the cells from the bacterial damage. Although antiseptics have a lower potency to induce bacterial resistance than antibiotics, concerns have been expressed regarding the overuse of antiseptics and the possible emergence of bacterial adaptation. Hence, an experimental system using microplate-laser-nephelometry was employed to test the adaptation capacity of Staphylococcus aureus during repeated treatment with polihexanide.
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