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In vitro germination and viability of pollen grains of banana diploids

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Author(s): Taliane Leila Soares | Sebastião de Oliveira e Silva | Maria Angélica Pereira de Carvalho Costa | Janay Almeida dos Santos-Serejo | Antônio da Silva Souza | Lucymeire Souza Morais Lino | Everton Hilo de Souza | Onildo Nunes de Jesus

Journal: Crop Breeding and Applied Biotechnology
ISSN 1518-7853

Volume: 8;
Issue: 2;
Start page: 111;
Date: 2008;
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Keywords: Musa acuminata | diploids | genetic breeding | pollen tube | culture medium.

ABSTRACT
The objective of this study was to evaluate the influence of pH on in vitro germination and pollen grain viabilityof banana diploids (AA) generated by the breeding program of Embrapa Mandioca e Fruticultura Tropical. The pollen grainswere inoculated in culture medium containing 15% sucrose, 0.01% H3BO3, 0.01% KNO3, 0.03% Ca(NO3)2.4H2O, 0.02%MgSO4.7H2O, solidified with 0.8% agar and pH adjusted to 5.8 or 7.0. Pollen viability was evaluated by staining with 1%acetic carmine. The germination percentages of the genotypes 9187-01 (90.0%) and M-53 (89.7%) in pH 7.0 medium werehighest, while the pollen tube length of genotype 9187-01 was approximately half the size (1.79mm) of genotype M-53(3.84mm). The pollen viability of the genotypes evaluated was higher than 85%, even for the diploids with a low in vitrogermination percentage.
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