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In vitro Inhibition of Mastitis Pathogens by Bacteriocin RN 86 Produced by an Indigenous Lactobacillus casei Isolate

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Author(s): Naheed Mojgani | Mehdi Perveen Ashtiani

Journal: Journal of Applied Sciences
ISSN 1812-5654

Volume: 6;
Issue: 12;
Start page: 2629;
Date: 2006;
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Keywords: Bovine mastitis | L. casei | bacteriocins | bactericidal | antibiotics | teat seals | in vitro

ABSTRACT
A broad-spectrum bacteriocin produced by L. casei RN 86 was a stable and relatively small molecule (5.5 KDa) being bactericidal againsta range of mastitis-causing bacterial pathogens. The antimicrobial activity of the peptide against the sensitive cells was 10 fold increased (AU mL-1) after partial purification by 20% ammonium sulphate precipitation. The partially purified peptide indicated to be bactericidal against S. aureus and S. agalactiae cells and killed approximately 106 to 109 of the actively growing sensitive cells within an hour of incubation. It caused rapid cessation of the growth of the sensitive strains within few minutes of contact, while almost 99.9% of the cells were killed within an hour of incubation with 10240 AU mL-1 of its concentrations. Owing to the wide spectrum activity, efforts were made to investigatethe in vitro efficacy of this antibacterial compound in combination with a teat seal formulation, against mastitis pathogens. A new prototype formulation containing the bacteriocin RN 86 with or without minimum concentrations of antibiotics and tween 80, which were the active ingredients of a teat seal used for the treatment of bovine mastitis. The results showed synergistic effect of all three components in combination and were the most effective in controlling the growth of Staphylococcus aureus and Streptococcus agalactiae during in vitro experiments.

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