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The In vitro Inhibitory Effects of Some Disinfectants on Enzyme Activity of Carbonic Anhydrase from Rainbow Trout (Oncorhynchus Mykiss) Gills

Author(s): ?zer Ayik | Olcay Hisar | Metin B?lb?l | ??kriye Aras Hisar | Telat Yanik

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 4;
Issue: 7;
Start page: 645;
Date: 2005;
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Keywords: Disinfectants | fish | inhibition | carbonic anhydrase

Traditional treatments of parasitic and bacterial disease in aquaculture are based on chemotherapeutic compounds. Although, a lot of compounds are used on fish treatment, their undesirable effects are not known in detail. In this study, the effects of some disinfectants - malachite green, methylene blue, potassium permanganate, chloramine-T, copper sulphate and formalin - on Rainbow Trout (RT) gill Carbonic Anhydrase (CA) which plays a key role in gas exchange, acid-base balance, osmoregulation and ionoregulation were investigated in vitro. For this purpose, CA was purified from RT gills by using sepharose-4$-L tyrosine-sulfanylamide affinity gel chromatography method at initial. CA enzyme with 55.56 (EU/mg proteins) specific activity was purified with a yield of 40 % and 104.8-fold finally. I (inhibition) values of malachite green, 50 methylene blue, potassium permanganate, chloramine-T and copper sulphate were determined as 0.05 mM, 0.023 mM, 0.15 mM, 0.32 mM and 5.39nM by means of activity % [disinfectant] graphs, respectively. In conclusion our data showed that all the disinfectants except formalin had the in vitro inhibitory effects on the rainbow trout gill CA enzyme whose inhibition could be hazardous to physiological functions such as osmoregulation and acid-base balance in fish.

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