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In Vitro Study to determine the effect of zinc on non-heme iron absorption

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Author(s): Aswir Abd Rashed

Journal: International Journal of Collaborative Research on Internal Medicine & Public Health
ISSN 1840-4529

Volume: 3;
Issue: 3;
Start page: 251;
Date: 2011;
Original page

Keywords: Iron uptake | Caco-2 TC& cells | Zinc | Ascorbic acid | Ferric iron

ABSTRACT
It has been well documented that bioavailability of iron is influenced by other food constituents. Wecarried out a kinetic study to look at the effect of zinc on ferric iron uptake in human epithelial Caco-2TC7 cell-line. Zinc reduced iron uptake in a concentration-dependent manner. Iron uptake wassignificantly increased in the presence of ascorbic acid in the medium. We also found that zincsignificantly increased the main iron transporter Divalent Metal Transporter-1 (DMT1) expression inwhole cell and membrane proteins. The evidence from in vitro studies show that zinc suppressed ironuptake by Caco-2 cells and this phenomenon could be reversed by the presence of ascorbic acid in thegrowth media.
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