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Whole Cell Based Electrochemical Biosensor for Monitoring Lead ions in Milk

Author(s): Neelam Verma | Hardeep Kaur | Sachin Kumar

Journal: Biotechnology
ISSN 1682-296X

Volume: 10;
Issue: 3;
Start page: 259;
Date: 2011;
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Keywords: lead specific column | NADPH oxidation | Electrochemical biosensor | microbial urease | Carbon paste electrode

Rapid industrialization, urbanization and modern agricultural practices have led to cumulative pollution of environment by heavy metals, thereby deteriorating water and food product quality. Milk and its products are most prone to heavy metal contamination and lead has been considered as a major contaminant in milk. A whole cell electrochemical lead biosensor has been developed using urease producing Bacillus sphaericus isolate (MTCC 5100) which was grown at 30C for 24 h and then harvested in phosphate buffer saline. Urease enzyme units available in the biomass were 53.871.41 IU mL-1. Whole cells were immobilized at the tip of carbon paste electrode and unutilized NADPH oxidation has been studied to reveal the lead concentration in milk. Preconcentration and specificity has been achieved by lead specific column. Lead analysis has been done at 25C in 5 min response time. Detection limit achieved is 2.4x10-3 nM well below the permissible limit of lead in milk. The developed method could be used for off line analysis of lead by portable handheld potentiostat.

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