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Yeast-Derived Avian Influenza Virus Hemagglutinin Protein Induced Immune Response in SPF Chicken

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Author(s): Hongzhuan Wu | Karyn Scissum-Gunn | Narendra K. Singh | Shan-Chia Ou | Joseph J. Giambrone

Journal: Journal of Animal and Veterinary Advances
ISSN 1680-5593

Volume: 10;
Issue: 8;
Start page: 999;
Date: 2011;
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Keywords: H10N7 | influenza | vaccine | yeast | chicken | USA

ABSTRACT
Influenza pandemic virus appears to have originated from reassortment of inherited genes (HA, NA and PB1) from the avian influenza pool and genes from the human influenza virus. Specifically, regarding the low pathogenic Avian Influenza Virus (AIV), there is no commercial vaccine yet available. Hence, it is a priority to develop alow pathogenic avian influenza vaccine to help reduce morbidity and mortality outcomes during the next pandemic flu outbreak. In this study, the HA gene of an AIV A/Northern shoreler/AL/26/2006 isolate, AIV subtype H10N7 was expressed in the yeast Saccharomyces pombe and the recombinant protein was used to immunize Specific Pathogen Free (SPF) leghorn chickens held in Horsfall isolation units via the upper respiratory route at 1, 7 and 14 days of age. Serum and spleens of immunized chickens were analyzed and the results demonstrate that the yeast-derived recombinant HA protein is as effective as inactivated vaccine in inducing Hemagglutination Inhibition (HI) antibody, neutralization antibody and a Th1 like cytokine immune response. The results suggest that the recombinant HA protein could be a promising new subunit vaccine agent against low pathogenic AIV in chicken.
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