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THE YIELD OF DNA IN THERMAL TERATED DEER MEAT

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Author(s): Ľubomir Belej | Miroslava Barnová | Lenka Maršálková | Jozef Golian

Journal: Potravinarstvo : Scientific Journal for Food Industry
ISSN 1338-0230

Volume: 5;
Issue: 3;
Start page: 6;
Date: 2011;
Original page

Keywords: DNA | temperature | deer meat

ABSTRACT
Residuals of DNA are one of the most important factors for detection, traceability and reverse authentication of deer meat. In this project we isolated DNA from deer processed meat and analysed by electrophoresis. Goal of the study was compute ratio between raw meat and several heat processed deer meat. Samples were prepared by five heat treatment techniques (pan roasted with temperature 180-240°C, fried with 156°C, braised with temperature 100-150°C, boiled in 100.2°C water and autoclaved in different time intervals). The highest amount of residual DNA 1927ng was obtained with two hours boiled sample. The lowest value 89.89ng was obtained with one hour braised sample. In technological adjustments highest amount of DNA and 1927ng, so the total yield of 192.7ng.-l was observed in the sample we cooked for two hours at boiling temperature.   doi:10.5219/153 
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