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ZAK negatively regulates RhoGDIβ-induced Rac1-mediated hypertrophic growth and cell migration

Author(s): Huang Chih-Yang | Yang Li-Chiu | Liu Kuan-Yu | Chang I-Chang | Liao Pao-Hsin | Chou Janet | Chou Ming-Yung | Lin Wei-Wen | Yang Jaw-Ji

Journal: Journal of Biomedical Science
ISSN 1021-7770

Volume: 16;
Issue: 1;
Start page: 56;
Date: 2009;
Original page

Abstract RhoGDIβ, a Rho GDP dissociation inhibitor, induced hypertrophic growth and cell migration in a cultured cardiomyoblast cell line, H9c2. We demonstrated that RhoGDIβ plays a previously undefined role in regulating Rac1 expression through transcription to induce hypertrophic growth and cell migration and that these functions are blocked by the expression of a dominant-negative form of Rac1. We also demonstrated that knockdown of RhoGDIβ expression by RNA interference blocked RhoGDIβ-induced Rac1 expression and cell migration. We demonstrated that the co-expression of ZAK and RhoGDIβ in cells resulted in an inhibition in the activity of ZAK to induce ANF expression. Knockdown of ZAK expression in ZAK-RhoGDIβ-expressing cells by ZAK-specific RNA interference restored the activities of RhoGDIβ.

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