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Monitoring of transcript regulation and protein production related stress responses in Pichia pastoris secreting Fab antibody fragments

Author(s): Gasser Brigitte | Maurer Michael | Sauer Michael | Saloheimo Markku | Rautio Jari | Penttila Merja | Mattanovich Diethard
Regulation of methanol utilisation pathway genes in yeasts

Author(s): Hartner Franz | Glieder Anton
A rational approach to optimize feed profiles for the maximization of productivity of secreted proteins expressed in Pichia pastoris

Author(s): Maurer Michael | Gasser Brigitte | Kühleitner Manfred | Mattanovich Diethard
Disruption of the GAS1 gene of Pichia pastoris confers a supersecretory phenotype for Rhizopus oryzae lipase, but not for human trypsinogen

Author(s): Marx Hans | Sauer Michael | Resina David | Vai Marina | Porro Danilo | Valero Francisco | Ferrer Pau | Mattanovich Diethard
Heterologous expression of isotopically labeled Trichoderma reesei tyrosinase 2 in Pichia pastoris

Author(s): Westerholm-Parvinen Ann | Mattinen Maija-Liisa | Selinheimo Emilia | Saloheimo Markku
Analysis of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated formaldehyde dehydrogenase promoter (PFLD1)

Author(s): Resina David | Cos Oriol | Gasser Brigitte | Mauer Michael | Marx Hans | Sauer Michael | Valero Francisco | Mattanovich Diethard | Ferrer Pau
Limitations using GFP as a protein expression reporter in Pichia pastoris

Author(s): Surribas Anna | Resina David | Ferrer Pau | Valero Francisco
Cloning and overexpression of a yeast phytase gene in Pichia pastoris

Author(s): Ragon Mélanie | Neugnot-Roux Virginie | Moulin Guy | Boze Hélène
Optimisation of substrate feeding in shake flask cultures of Pichia pastoris for recombinant protein production

Author(s): Bollok Monika | Ruottinen Maria | Krause Mirja | Vasala Antti | Hämäläinen Eija-Riitta | Neubauer Antje | Myllyharju Johanna | Neubauer Peter
Interfacing Pichia pastoris cultivation with expanded bed adsorption

Author(s): Jahic Mehmedalija | Knoblechner Josef | Charoenrat Theppanya | Enfors Sven-Olof | Veide Andres
Recombinant expression of monovalent and bivalent anti-TNT-antibodies – evaluation of different expression systems

Author(s): MLADEN SIMONOVIC | SVETLANA ZLATANOVIC-MILOSEVIC | MIROSLAV M. VRVIC | BRANISLAV SIMONOVIC
Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris

Author(s): Canales Mario | de la Lastra José | Naranjo Victoria | Nijhof Ard | Hope Michelle | Jongejan Frans | de la Fuente José
Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Author(s): Marx Hans | Mattanovich Diethard | Sauer Michael
Open access to sequence: Browsing the Pichia pastoris genome

Author(s): Mattanovich Diethard | Callewaert Nico | Rouzé Pierre | Lin Yao-Cheng | Graf Alexandra | Redl Andreas | Tiels Petra | Gasser Brigitte | De Schutter Kristof
Cloning and Expression of Aspergillus tamarii FS132 Lipase Gene in Pichia pastoris

Author(s): Bihong Shi | Liqing Zeng | Haolei Song | Qiaoqin Shi | Songgang Wu
Ribosome-Inactivating Proteins: From Plant Defense to Tumor Attack

Author(s): Maddalena de Virgilio | Alessio Lombardi | Rocco Caliandro | Maria Serena Fabbrini
Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Author(s): Jacobs Pieter | Inan Mehmet | Festjens Nele | Haustraete Jurgen | Van Hecke Annelies | Contreras Roland | Meagher Michael | Callewaert Nico
High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Author(s): Hartwig Daiane | Oliveira Thaís | Seixas Fabiana | Forster Karine | Rizzi Caroline | Hartleben Cláudia | McBride Alan | Dellagostin Odir
Large-scale functional expression of WT and truncated human adenosine A2A receptor in Pichia pastoris bioreactor cultures

Author(s): Singh Shweta | Gras Adrien | Fiez-Vandal Cédric | Ruprecht Jonathan | Rana Rohini | Martinez Magdalena | Strange Philip | Wagner Renaud | Byrne Bernadette
Addition of a sequence from α2-antiplasmin transforms human serum albumin into a blood clot component that speeds clot lysis

Author(s): Sheffield William | Eltringham-Smith Louise | Gataiance Sharon | Bhakta Varsha
Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: Application to intracellular production of Hepatitis B surface antigen

Author(s): Gurramkonda Chandrasekhar | Adnan Ahmad | Gäbel Thomas | Lünsdorf Heinrich | Ross Anton | Nemani Satish | Swaminathan Sathyamangalam | Khanna Navin | Rinas Ursula
Vaccination with recombinant Boophilus annulatus Bm86 ortholog protein, Ba86, protects cattle against B. annulatus and B. microplus infestations

Author(s): Canales Mario | Almazán Consuelo | Naranjo Victoria | Jongejan Frans | de la Fuente José
Optimizing expression of the pregnancy malaria vaccine candidate, VAR2CSA in Pichia pastoris

Author(s): Avril Marion | Hathaway Marianne | Cartwright Megan | Gose Severin | Narum David | Smith Joseph
Efficient production of human bivalent and trivalent anti-MUC1 Fab-scFv antibodies in Pichia pastoris

Author(s): Schoonooghe Steve | Kaigorodov Vladimir | Zawisza Monika | Dumolyn Caroline | Haustraete Jurgen | Grooten Johan | Mertens Nico
Cloning and constitutive expression of Deschampsia antarctica Cu/Zn superoxide dismutase in Pichia pastoris

Author(s): Sánchez-Venegas Jaime | Navarrete Alejandro | Dinamarca Jorge | Bravo Ramírez León | Moraga Ana | Gidekel Manuel
Cloning, expression in Pichia pastoris, and characterization of a thermostable GH5 mannan endo-1,4-β-mannosidase from Aspergillus niger BK01

Author(s): Bien-Cuong Do | Thi-Thu Dang | Berrin Jean-Guy | Haltrich Dietmar | Kim-Anh To | Sigoillot Jean-Claude | Yamabhai Montarop
Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at different oxygen levels

Author(s): Carnicer Marc | Baumann Kristin | Töplitz Isabelle | Sánchez-Ferrando Francesc | Mattanovich Diethard | Ferrer Pau | Albiol Joan
Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis

Author(s): van Zutphen Tim | Baerends Richard | Susanna Kim | de Jong Anne | Kuipers Oscar | Veenhuis Marten | van der Klei Ida
Cloning of the Zygosaccharomyces bailii GAS1 homologue and effect of cell wall engineering on protein secretory phenotype

Author(s): Passolunghi Simone | Riboldi Luca | Dato Laura | Porro Danilo | Branduardi Paola
Heterologous expression of leader-less pga gene in Pichia pastoris: intracellular production of prokaryotic enzyme

Author(s): Marešová Helena | Marková Zdena | Valešová Renáta | Sklenář Jan | Kyslík Pavel
Epitope mapping of PfCP-2.9, an asexual blood-stage vaccine candidate of Plasmodium falciparum

Author(s): Li Changling | Wang Rui | Wu Yuan | Zhang Dongmei | He Zhicheng | Pan Weiqing
Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin

Author(s): Gurramkonda Chandrasekhar | Polez Sulena | Skoko Natasa | Adnan Ahmad | Gäbel Thomas | Chugh Dipti | Swaminathan Sathyamangalam | Khanna Navin | Tisminetzky Sergio | Rinas Ursula
Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is improved by truncating the gene sequence

Author(s): Bai Yingguo | Wang Jianshe | Zhang Zhifang | Shi Pengjun | Luo Huiying | Huang Huoqing | Luo Chunliang | Yao Bin
Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Author(s): Abad Sandra | Nahalka Jozef | Bergler Gabriele | Arnold S Alison | Speight Robert | Fotheringham Ian | Nidetzky Bernd | Glieder Anton
The response to unfolded protein is involved in osmotolerance of Pichia pastoris

Author(s): Dragosits Martin | Stadlmann Johannes | Graf Alexandra | Gasser Brigitte | Maurer Michael | Sauer Michael | Kreil David | Altmann Friedrich | Mattanovich Diethard
The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins

Author(s): Guerfal Mouna | Ryckaert Stefan | Jacobs Pieter | Ameloot Paul | Van Craenenbroeck Kathleen | Derycke Riet | Callewaert Nico
Heterologous Expression of Human Granulocyte-Colony Stimulating Factor in Pichia pastoris

Author(s): Alireza Saeedinia | Mehdi Shamsara | Ali Bahrami | Mehdi Zeinoddini | Mohammad Ali Naseeri-Khalili | Reza Mohammadi | Narges Malek Sabet | Hosseinali Sami
Revisión de tema: Pichia pastoris, una alternativa para la producción de glicoproteínas humanas de uso terapéutico. Estrategias de fermentación

Author(s): Córdoba Ruiz Henry | Algecira Encizo Néstor | Poutou Piñales Raúl | Barrera Avellaneda Luis Alejandro
Glycosylation and pH stability of penicillin G acylase from providencia rettgeri produced in Pichia pastoris

Author(s): Šenerović Lidija | Stanković Nad | Ljubijankić G. | Vasiljević Branka
A multi-level study of recombinant Pichia pastoris in different oxygen conditions

Author(s): Baumann Kristin | Carnicer Marc | Dragosits Martin | Graf Alexandra | Stadlmann Johannes | Jouhten Paula | Maaheimo Hannu | Gasser Brigitte | Albiol Joan | Mattanovich Diethard | Ferrer Pau
Constitutive Expression of Thermobifida fusca Thermostable Acetylxylan Esterase Gene in Pichia pastoris

Author(s): Chao-Hsun Yang | Kun-I Lin | Gen-Hung Chen | Yu-Fen Chen | Cheng-Yu Chen | Wei-Lin Chen | Yu-Chun Huang
Induction of strain-transcendent antibodies to placental-type isolates with VAR2CSA DBL3 or DBL5 recombinant proteins

Author(s): Avril Marion | Cartwright Megan | Hathaway Marianne | Smith Joseph
A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination

Author(s): Vasconcelos Erico | Santana Celso | Godoy Claudia | Seixas Claudine | Silva Marilia | Moreira Leonora | Oliveira-Neto Osmundo | Price Daniel | Fitches Elaine | Filho Edivaldo | Mehta Angela | Gatehouse John | Grossi-De-Sa Maria
Purification and characterization of the first recombinant bird pancreatic lipase expressed in Pichia pastoris: The turkey

Author(s): Bou Ali Madiha | Ben Ali Yassine | Karray Aida | Fendri Ahmed | Gargouri Youssef
VEGF121b and VEGF165b are weakly angiogenic isoforms of VEGF-A

Author(s): Catena Raúl | Larzabal Leyre | Larrayoz Marta | Molina Eva | Hermida Jose | Agorreta Jackeline | Montes Ramon | Pio Ruben | Montuenga Luis | Calvo Alfonso
GH10 xylanase D from Penicillium funiculosum: biochemical studies and xylooligosaccharide production

Author(s): Lafond Mickael | Tauzin Alexandra | Desseaux Véronique | Bonnin Estelle | Ajandouz El-Hassan | Giardina Thierry
Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.)

Author(s): Ueno Keiji | Ishiguro Yojiro | Yoshida Midori | Onodera Shuichi | Shiomi Norio
Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

Author(s): Routledge Sarah | Hewitt Christopher | Bora Nagamani | Bill Roslyn
A visual method for direct selection of high-producing Pichia pastoris clones

Author(s): Hu Fan | Li Xin | Lü Jie | Mao Pei | Jin Xiang | Rao Ben | Zheng Peng | Zhou Yu | Liu Sheng | Ke Tao | Ma Xiang | Ma Li
Expression of Pisum sativum defensin 1 (Psd1) in shaking flasks and bioreactor cultivations of recombinant Pichia pastoris at different pHs

Author(s): Larentis A. L. | Almeida M. S. | Cabral K. M. S. | Medeiros L. N. | Kurtenbach E. | Coelho M. A. Z.
The evaluation of yeast derivatives as adjuvants for the immune response to the Bm86 antigen in cattle

Author(s): Rodríguez Valle Manuel | Montero Carlos | Machado Héctor | Joglar Marisdania
Temperature limited fed-batch technique for control of proteolysis in Pichia pastoris bioreactor cultures

Author(s): Jahic Mehmedalija | Wallberg Fredrik | Bollok Monika | Garcia Percival | Enfors Sven-Olof
Serological detection of Plasmodium vivax malaria using recombinant proteins corresponding to the 19-kDa C-terminal region of the merozoite surface protein-1

Author(s): Rodrigues Maria Helena | Cunha Maristela | Machado Ricardo | Ferreira Orlando | Rodrigues Mauricio | Soares Irene
Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Author(s): Sauer Michael | Branduardi Paola | Gasser Brigitte | Valli Minoska | Maurer Michael | Porro Danilo | Mattanovich Diethard
A new wireless system for decentralised measurement of physiological parameters from shake flasks

Author(s): Vasala Antti | Panula Johanna | Bollók Monika | Illmann Lutz | Hälsig Christian | Neubauer Peter
High expression of human carboxypeptidase M in Pichia pastoris: Purification and partial characterization

Author(s): Craveiro R.B. | Ramalho J.D. | Chagas J.R. | Wang P.H.M. | Casarini D.E. | Pesquero J.L. | Araújo R.C. | Pesquero J.B.
Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enzyme

Author(s): Colao Maria | Lupino Stefania | Garzillo Anna | Buonocore Vincenzo | Ruzzi Maurizio
Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pichia pastoris

Author(s): Maurer Michael | Kühleitner Manfred | Gasser Brigitte | Mattanovich Diethard
Single chain Fab (scFab) fragment

Author(s): Hust Michael | Jostock Thomas | Menzel Christian | Voedisch Bernd | Mohr Anja | Brenneis Mariam | Kirsch Martina | Meier Doris | Dübel Stefan
Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

Author(s): Gasser Brigitte | Maurer Michael | Rautio Jari | Sauer Michael | Bhattacharyya Anamitra | Saloheimo Markku | Penttilä Merja | Mattanovich Diethard
Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induction

Author(s): Resina David | Bollók Mónika | Khatri Narendar | Valero Francisco | Neubauer Peter | Ferrer Pau
Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system

Author(s): Ruottinen Maria | Bollok Monika | Kögler Martin | Neubauer Antje | Krause Mirja | Hämäläinen Eija-Riitta | Myllyharju Johanna | Vasala Antti | Neubauer Peter
Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays

Author(s): Graf Alexandra | Gasser Brigitte | Dragosits Martin | Sauer Michael | Leparc Germán | Tüchler Thomas | Kreil David | Mattanovich Diethard
Producción de una fitasa recombinante en Pichia pastoris

Author(s): Martha Guerrero Olazar n | Lil Rodr guez Blanco | Jos Ma. Viader Salvad
A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains

Author(s): Dietzsch Christian | Spadiut Oliver | Herwig Christoph
Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris

Author(s): Baumann Kristin | Adelantado Núria | Lang Christine | Mattanovich Diethard | Ferrer Pau
Optimal Production and Biochemical Properties of a Lipase from Candida albicans

Author(s): Dongming Lan | Shulin Hou | Ning Yang | Chris Whiteley | Bo Yang | Yonghua Wang
Molecular cloning and expression of a synthetic gene encoding a ?-glucosidase of Aspergillus niger in the methylotrophic yeast Pichia pastoris

Author(s): Jean Louis Didier MEKOO | Yanan YANG | Yanan YANG | Taiming Li | Yanan YANG | Taiming Li | Rongyue CAO | Taiming Li | Jingjing LIU | Rongyue CAO | Rongyue CAO | Jingjing LIU | Jingjing LIU
Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

Author(s): Asada Hidetsugu | Uemura Tomoko | Yurugi-Kobayashi Takami | Shiroishi Mitsunori | Shimamura Tatsuro | Tsujimoto Hirokazu | Ito Keisuke | Sugawara Taishi | Nakane Takanori | Nomura Norimichi | Murata Takeshi | Haga Tatsuya | Iwata So | Kobayashi Takuya
The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis

Author(s): Baumann Kristin | Dato Laura | Graf Alexandra | Frascotti Gianni | Dragosits Martin | Porro Danilo | Mattanovich Diethard | Ferrer Pau | Branduardi Paola
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

Author(s): Nordén Kristina | Agemark Maria | Danielson Jonas | Alexandersson Erik | Kjellbom Per | Johanson Urban
Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling procedure

Author(s): Pfeffer Martin | Maurer Michael | Köllensperger Gunda | Hann Stephan | Graf Alexandra | Mattanovich Diethard
A Novel Cold-Active Lipase from Candida albicans: Cloning, Expression and Characterization of the Recombinant Enzyme

Author(s): Dong-Ming Lan | Ning Yang | Wen-Kai Wang | Yan-Fei Shen | Bo Yang | Yong-Hua Wang

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