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BMC Biotechnology

ISSN: 1472--6750
Publisher: BioMed Central


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Evaluating target silencing by short hairpin RNA mediated by the group I intron in cultured mammalian cells

Author(s): Noguchi Kousei | Ishitu Yoshio | Takaku Hiroshi
Volume: 11
Issue: 1
Year: 2011
Hydroxylation of recombinant human collagen type I alpha 1 in transgenic maize co-expressed with a recombinant human prolyl 4-hydroxylase

Author(s): Xu Xing | Gan Qinglei | Clough Richard | Pappu Kameshwari | Howard John | Baez Julio | Wang Kan
Volume: 11
Issue: 1
Year: 2011
T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis

Author(s): Viollet Sebastien | Fuchs Ryan | Munafo Daniela | Zhuang Fanglei | Robb Gregory
Volume: 11
Issue: 1
Year: 2011
Expression of tung tree diacylglycerol acyltransferase 1 in E. coli

Author(s): Cao Heping | Chapital Dorselyn | Shockey Jay | Klasson K Thomas
Volume: 11
Issue: 1
Year: 2011
RNA interference in marine and freshwater sponges: actin knockdown in Tethya wilhelma and Ephydatia muelleri by ingested dsRNA expressing bacteria

Author(s): Rivera Ajna | Hammel Jörg | Haen Karri | Danka Elizabeth | Cieniewicz Brandon | Winters Ian | Posfai Dora | Wörheide Gert | Lavrov Dennis | Knight Scott | Hill Malcolm | Hill April | Nickel Michael
Volume: 11
Issue: 1
Year: 2011
Immobilized Rhizopus oryzae lipase catalyzed synthesis of palm stearin and cetyl alcohol wax esters: Optimization by Response Surface Methodology

Author(s): Sellami Mohamed | Aissa Imen | Frikha Fakher | Gargouri Youssef | Miled Nabil
Volume: 11
Issue: 1
Year: 2011
An intein with genetically selectable markers provides a new approach to internally label proteins with GFP

Author(s): Ramsden Richard | Arms Luther | Davis Trisha | Muller Eric
Volume: 11
Issue: 1
Year: 2011
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers

Author(s): Balcells Ingrid | Cirera Susanna | Busk Peter
Volume: 11
Issue: 1
Year: 2011
A novel subtilase with NaCl-activated and oxidant-stable activity from Virgibacillus sp. SK37

Author(s): Phrommao Ekkarat | Yongsawatdigul Jirawat | Rodtong Sureelak | Yamabhai Montarop
Volume: 11
Issue: 1
Year: 2011
Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein

Author(s): Riihimäki Tiina | Hiltunen Soili | Rangl Martina | Nordlund Henri | Määttä Juha | Ebner Andreas | Hinterdorfer Peter | Kulomaa Markku | Takkinen Kristiina | Hytönen Vesa
Volume: 11
Issue: 1
Year: 2011
Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing

Author(s): Lutz Kerry | Wang Wenqin | Zdepski Anna | Michael Todd
Volume: 11
Issue: 1
Year: 2011
Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor

Author(s): Dmytruk Kostyantyn | Smutok Oleh | Dmytruk Olena | Schuhmann Wolfgang | Sibirny Andriy
Volume: 11
Issue: 1
Year: 2011
Purification of phage display-modified bacteriophage T4 by affinity chromatography

Author(s): Oślizło Anna | Miernikiewicz Paulina | Piotrowicz Agnieszka | Owczarek Barbara | Kopciuch Agnieszka | Figura Grzegorz | Dąbrowska Krystyna
Volume: 11
Issue: 1
Year: 2011
Antibodies on demand: a fast method for the production of human scFvs with minimal amounts of antigen

Author(s): Babel Ingrid | Barderas Rodrigo | Peláez-García Alberto | Casal J Ignacio
Volume: 11
Issue: 1
Year: 2011
Cell-free synthesis of a functional G protein-coupled receptor complexed with nanometer scale bilayer discs

Author(s): Yang Jian-Ping | Cirico Tatiana | Katzen Federico | Peterson Todd | Kudlicki Wieslaw
Volume: 11
Issue: 1
Year: 2011
Evaluation of biolistic gene transfer methods in vivo using non-invasive bioluminescent imaging techniques

Author(s): Xia Jixiang | Martinez Angela | Daniell Henry | Ebert Steven
Volume: 11
Issue: 1
Year: 2011
Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating

Author(s): Ren Yuhong | Rivera Jose | He Lihong | Kulkarni Harsha | Lee Dong-Keun | Messersmith Phillip
Volume: 11
Issue: 1
Year: 2011
Construction of high quality Gateway™ entry libraries and their application to yeast two-hybrid for the monocot model plant Brachypodium distachyon

Author(s): Cao Shuanghe | Siriwardana Chamindika | Kumimoto Roderick | Holt Ben
Volume: 11
Issue: 1
Year: 2011
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

Author(s): Nordén Kristina | Agemark Maria | Danielson Jonas | Alexandersson Erik | Kjellbom Per | Johanson Urban
Volume: 11
Issue: 1
Year: 2011
Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration

Author(s): Zimmermann Katrin | Scheibe Oliver | Kocourek Andreas | Muelich Jutta | Jurkiewicz Elke | Pfeifer Alexander
Volume: 11
Issue: 1
Year: 2011
Identification of a novel temperature sensitive promoter in cho cells

Author(s): Thaisuchat Haruthai | Baumann Martina | Pontiller Jens | Hesse Friedemann | Ernst Wolfgang
Volume: 11
Issue: 1
Year: 2011
Echium acanthocarpum hairy root cultures, a suitable system for polyunsaturated fatty acid studies and production

Author(s): Cequier-Sánchez Elena | Rodríguez Covadonga | Dorta-Guerra Roberto | Ravelo Ángel | Zárate Rafael
Volume: 11
Issue: 1
Year: 2011
A visible, targeted high-efficiency gene delivery and transfection strategy

Author(s): Yuan Qiao-Ying | Huang Jing | Chu Bao-Cheng | Li Xing-Sheng | Si Liang-Yi
Volume: 11
Issue: 1
Year: 2011
Sequencing bias: comparison of different protocols of MicroRNA library construction

Author(s): Tian Geng | Gan ShangQuang | Yin XuYang | Luo Hong | Xu XiaoHong | Bolund Lars | Zhang XiuQing | Li Ning
Volume: 11
Issue: 1
Year: 2011
Efficient production of human acidic fibroblast growth factor in pea (Pisum sativum L.) plants by agroinfection of germinated seeds

Author(s): Fan Yajun | Li Wei | Wang Junjie | Liu Jingying | Yang Meiying | Xu Duo | Zhu Xiaojuan | Wang Xingzhi
Volume: 11
Issue: 1
Year: 2011
Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots

Author(s): Kai Guoyin | Yang Sheng | Luo Xiuqin | Zhou Wentao | Fu Xueqing | Zhang Ang | Zhang Yan | Xiao Jianbo
Volume: 11
Issue: 1
Year: 2011
Potential errors in conventional DOT measurement techniques in shake flasks and verification using a rotating flexitube optical sensor

Author(s): Hansen Sven | Kensy Frank | Käser Andreas | Büchs Jochen
Volume: 11
Issue: 1
Year: 2011
mRNA transcript quantification in archival samples using multiplexed, color-coded probes

Author(s): Reis Patricia | Waldron Levi | Goswami Rashmi | Xu Wei | Xuan Yali | Perez-Ordonez Bayardo | Gullane Patrick | Irish Jonathan | Jurisica Igor | Kamel-Reid Suzanne
Volume: 11
Issue: 1
Year: 2011
1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

Author(s): Horáková Helena | Polakovičová Iva | Shaik Gouse | Eitler Jiří | Bugajev Viktor | Dráberová Lubica | Dráber Petr
Volume: 11
Issue: 1
Year: 2011
Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies

Author(s): Cheung Stanley | Liu Li-zhong | Lan Lin-lin | Liu Qiao-quan | Sun Samuel | Chan Juliana | Tong Peter
Volume: 11
Issue: 1
Year: 2011
Estimation of affinities of ligands in mixtures via magnetic recovery of target-ligand complexes and chromatographic analyses: chemometrics and an experimental model

Author(s): Yang Xiaolan | Xie Yanling | Pu Jun | Zhao Hua | Liao Juan | Yuan Yonghua | Zhu Sha | Long Gaobo | Zhang Chun | Yuan Huidong | Chen Yiwen | Liao Fei
Volume: 11
Issue: 1
Year: 2011
Supramolecular assemblies of rifampicin and cationic bilayers: preparation, characterization and micobactericidal activity

Author(s): Barbassa Lilian | Mamizuka Elsa | Carmona-Ribeiro Ana
Volume: 11
Issue: 1
Year: 2011
Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system

Author(s): Nozawa Akira | Ogasawara Tomio | Matsunaga Satoko | Iwasaki Takahiro | Sawasaki Tatsuya | Endo Yaeta
Volume: 11
Issue: 1
Year: 2011
Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells

Author(s): Kurosawa Nobuyuki | Yoshioka Megumi | Isobe Masaharu
Volume: 11
Issue: 1
Year: 2011
The generation and evaluation of recombinant human IgA specific for Plasmodium falciparum merozoite surface protein 1-19 (PfMSP119)

Author(s): Shi Jianguo | McIntosh Richard | Adame-Gallegos Jaime | Dehal Prabhjyot | van Egmond Marjolein | van de Winkel Jan | Draper Simon | Forbes Emily | Corran Patrick | Holder Anthony | Woof Jenny | Pleass Richard
Volume: 11
Issue: 1
Year: 2011
Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells

Author(s): Chen Allen | Poh Swan Li | Dietzsch Christian | Roethl Elisabeth | Yan Mylene | Ng Say Kong
Volume: 11
Issue: 1
Year: 2011
Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation

Author(s): Mann David | King Zachary | Liu Wusheng | Joyce Blake | Percifield Ryan | Hawkins Jennifer | LaFayette Peter | Artelt Barbara | Burris Jason | Mazarei Mitra | Bennetzen Jeffrey | Parrott Wayne | Stewart Charles
Volume: 11
Issue: 1
Year: 2011
Site-specific modification of ED-B-targeting antibody using intein-fusion technology

Author(s): Möhlmann Sina | Bringmann Peter | Greven Simone | Harrenga Axel
Volume: 11
Issue: 1
Year: 2011
Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

Author(s): Burleigh Susan | van de Laar Teun | Stroop Corné | van Grunsven Wout | O'Donoghue Niaobh | Rudd Pauline | Davey Gavin
Volume: 11
Issue: 1
Year: 2011
Do-it-yourself: construction of a custom cDNA macroarray platform with high sensitivity and linear range

Author(s): Boonefaes Tom | Houthuys Erica | Van den Bergh Rafael | Vander Beken Seppe | Raes Geert | Brouckaert Peter | De Baetselier Patrick | Grooten Johan
Volume: 11
Issue: 1
Year: 2011
Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential

Author(s): Bandounas Luaine | Wierckx Nick | de Winde Johannes | Ruijssenaars Harald
Volume: 11
Issue: 1
Year: 2011
Ubiquitin fusion expression and tissue-dependent targeting of hG-CSF in transgenic tobacco

Author(s): Tian Li | Sun Samuel
Volume: 11
Issue: 1
Year: 2011
FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

Author(s): Li Chaokun | Wen Aiyun | Shen Benchang | Lu Jia | Huang Yao | Chang Yongchang
Volume: 11
Issue: 1
Year: 2011
Regeneration of multiple shoots from transgenic potato events facilitates the recovery of phenotypically normal lines: assessing a cry9Aa2 gene conferring insect resistance

Author(s): Meiyalaghan Sathiyamoorthy | Barrell Philippa | Jacobs Jeanne | Conner Anthony
Volume: 11
Issue: 1
Year: 2011
Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers

Author(s): Sa-ngiamsuntorn Khanit | Wongkajornsilp Adisak | Kasetsinsombat Kanda | Duangsa-ard Sunisa | Nuntakarn Lalana | Borwornpinyo Suparerk | Akarasereenont Pravit | Limsrichamrern Somchai | Hongeng Suradej
Volume: 11
Issue: 1
Year: 2011
Design and development of a peptide-based adiponectin receptor agonist for cancer treatment

Author(s): Otvos Laszlo | Haspinger Eva | La Russa Francesca | Maspero Federica | Graziano Patrizia | Kovalszky Ilona | Lovas Sandor | Nama Kaushik | Hoffmann Ralf | Knappe Daniel | Cassone Marco | Wade John | Surmacz Eva
Volume: 11
Issue: 1
Year: 2011
Isolation of a Highly Thermal Stable Lama Single Domain Antibody Specific for Staphylococcus aureus Enterotoxin B

Author(s): Graef Russell | Anderson George | Doyle Katherine | Zabetakis Dan | Sutton Felicia | Liu Jinny | Serrano-González Joseline | Goldman Ellen | Cooper Lynn
Volume: 11
Issue: 1
Year: 2011
Nuclease-resistant immunostimulatory phosphodiester CpG oligodeoxynucleotides as human Toll-like receptor 9 agonists

Author(s): Meng Wenjun | Yamazaki Tomohiko | Nishida Yuuki | Hanagata Nobutaka
Volume: 11
Issue: 1
Year: 2011
Directed PCR-free engineering of highly repetitive DNA sequences

Author(s): Scior Annika | Preissler Steffen | Koch Miriam | Deuerling Elke
Volume: 11
Issue: 1
Year: 2011
Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase

Author(s): Ruiz Oscar | Alvarez Derry | Gonzalez-Ruiz Gloriene | Torres Cesar
Volume: 11
Issue: 1
Year: 2011
Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis)

Author(s): Oliveira Gustavo | Silva Maria | Lucena Wagner | Nakasu Erich | Firmino Alexandre | Beneventi Magda | Souza Djair | Gomes José | de Souza José | Rigden Daniel | Ramos Hudson | Soccol Carlos | Grossi-de-Sa Maria
Volume: 11
Issue: 1
Year: 2011
Metabolic and Kinetic analyses of influenza production in perfusion HEK293 cell culture

Author(s): Petiot Emma | Jacob Danielle | Lanthier Stephane | Lohr Verena | Ansorge Sven | Kamen Amine
Volume: 11
Issue: 1
Year: 2011
RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers

Author(s): Dobosy Joseph | Rose Scott | Beltz Kristin | Rupp Susan | Powers Kristy | Behlke Mark | Walder Joseph
Volume: 11
Issue: 1
Year: 2011
Sustained productivity in recombinant Chinese Hamster Ovary (CHO) cell lines: proteome analysis of the molecular basis for a process-related phenotype

Author(s): Meleady Paula | Doolan Padraig | Henry Michael | Barron Niall | Keenan Joanne | O'Sullivan Finbar | Clarke Colin | Gammell Patrick | Melville Mark | Leonard Mark | Clynes Martin
Volume: 11
Issue: 1
Year: 2011
Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR

Author(s): Roberts Ian | Ng Grace | Foster Nicola | Stanley Margaret | Herdman Michael | Pett Mark | Teschendorff Andrew | Coleman Nicholas
Volume: 8
Issue: 1
Year: 2008
Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure

Author(s): Minaeva Natalia | Gak Evgeny | Zimenkov Danila | Skorokhodova Aleksandra | Biryukova Irina | Mashko Sergey
Volume: 8
Issue: 1
Year: 2008
Detection of N-acyl homoserine lactones using a traI-luxCDABE-based biosensor as a high-throughput screening tool

Author(s): Bernier Steve | Beeston Anne | Sokol Pamela
Volume: 8
Issue: 1
Year: 2008
Comparison of the mismatch-specific endonuclease method and denaturing high-performance liquid chromatography for the identification of HBB gene mutations

Author(s): Hung Chia-Cheng | Su Yi-Ning | Lin Chia-Yun | Chang Yin-Fei | Chang Chien-Hui | Cheng Wen-Fang | Chen Chi-An | Lee Chien-Nan | Lin Win-Li
Volume: 8
Issue: 1
Year: 2008
Display of a Maize cDNA library on baculovirus infected insect cells

Author(s): Meller Harel Helene | Fontaine Veronique | Chen Hongying | Jones Ian | Millner Paul
Volume: 8
Issue: 1
Year: 2008
Analysis of transient phosphorylation-dependent protein-protein interactions in living mammalian cells using split-TEV

Author(s): Wehr Michael | Reinecke Lisa | Botvinnik Anna | Rossner Moritz
Volume: 8
Issue: 1
Year: 2008
Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells

Author(s): Witusik Monika | Piaskowski Sylwester | Hulas-Bigoszewska Krystyna | Zakrzewska Magdalena | Gresner Sylwia | Azizi S | Krynska Barbara | Liberski Pawel | Rieske Piotr
Volume: 8
Issue: 1
Year: 2008
Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cells

Author(s): D'Aiuto Leonardo | Robison Clinton | Gigante Margherita | Nwanegbo Edward | Shaffer Benjamin | Sukhwani Meena | Castro Carlos | Chaillet J Richard
Volume: 8
Issue: 1
Year: 2008
Expression of HIV-1 antigens in plants as potential subunit vaccines

Author(s): Meyers Ann | Chakauya Ereck | Shephard Enid | Tanzer Fiona | Maclean James | Lynch Alisson | Williamson Anna-Lise | Rybicki Edward
Volume: 8
Issue: 1
Year: 2008
Three-dimensional culture of human meniscal cells: Extracellular matrix and proteoglycan production

Author(s): Gruber Helen | Mauerhan David | Chow Yin | Ingram Jane | Norton H James | Hanley Edward | Sun Yubo
Volume: 8
Issue: 1
Year: 2008
Impact of point-mutations on the hybridization affinity of surface-bound DNA/DNA and RNA/DNA oligonucleotide-duplexes: Comparison of single base mismatches and base bulges

Author(s): Naiser Thomas | Ehler Oliver | Kayser Jona | Mai Timo | Michel Wolfgang | Ott Albrecht
Volume: 8
Issue: 1
Year: 2008
Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production

Author(s): Schulze-Horsel Josef | Genzel Yvonne | Reichl Udo
Volume: 8
Issue: 1
Year: 2008
Substantially improved pharmacokinetics of recombinant human butyrylcholinesterase by fusion to human serum albumin

Author(s): Huang Yue-Jin | Lundy Paul | Lazaris Anthoula | Huang Yue | Baldassarre Hernan | Wang Bin | Turcotte Carl | Côté Mélanie | Bellemare Annie | Bilodeau Annie | Brouillard Sandra | Touati Madjid | Herskovits Peter | Bégin Isabelle | Neveu Nathalie | Brochu Eric | Pierson Janice | Hockley Duncan | Cerasoli Douglas | Lenz David | Wilgus Harvey | Karatzas Costas | Langermann Solomon
Volume: 8
Issue: 1
Year: 2008
RNAi-mediated gene silencing in tick synganglia: A proof of concept study

Author(s): Karim Shahid | Kenny Bronwyn | Troiano Emily | Mather Thomas
Volume: 8
Issue: 1
Year: 2008
Hydroxycamptothecin-loaded nanoparticles enhance target drug delivery and anticancer effect

Author(s): Wang Anxun | Li Su
Volume: 8
Issue: 1
Year: 2008
Differential protein expression following low temperature culture of suspension CHO-K1 cells

Author(s): Kumar Niraj | Gammell Patrick | Meleady Paula | Henry Michael | Clynes Martin
Volume: 8
Issue: 1
Year: 2008
Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections

Author(s): Panseri Silvia | Cunha Carla | Lowery Joseph | Del Carro Ubaldo | Taraballi Francesca | Amadio Stefano | Vescovi Angelo | Gelain Fabrizio
Volume: 8
Issue: 1
Year: 2008
Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice

Author(s): Williams Jason | Schray Rebecca | Sirsi Shashank | Lutz Gordon
Volume: 8
Issue: 1
Year: 2008
In vivo site-specific biotinylation of proteins within the secretory pathway using a single vector system

Author(s): Predonzani Andrea | Arnoldi Francesca | López-Requena Alejandro | Burrone Oscar
Volume: 8
Issue: 1
Year: 2008
Systolic and diastolic ventricular function in zebrafish embryos: Influence of norepenephrine, MS-222 and temperature

Author(s): Denvir Martin | Tucker Carl | Mullins John
Volume: 8
Issue: 1
Year: 2008
Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography

Author(s): Sewekow Eva | Keßler Lars | Seidel-Morgenstern Andreas | Rothkötter Hermann-Josef
Volume: 8
Issue: 1
Year: 2008
Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system

Author(s): Ruottinen Maria | Bollok Monika | Kögler Martin | Neubauer Antje | Krause Mirja | Hämäläinen Eija-Riitta | Myllyharju Johanna | Vasala Antti | Neubauer Peter
Volume: 8
Issue: 1
Year: 2008
A set of ligation-independent in vitro translation vectors for eukaryotic protein production

Author(s): Bardóczy Viola | Géczi Viktória | Sawasaki Tatsuya | Endo Yaeta | Mészáros Tamás
Volume: 8
Issue: 1
Year: 2008
Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms

Author(s): Buh Gašparič Meti | Cankar Katarina | Žel Jana | Gruden Kristina
Volume: 8
Issue: 1
Year: 2008
Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection

Author(s): Goffinet Marine | Chinestra Patrick | Lajoie-Mazenc Isabelle | Medale-Giamarchi Claire | Favre Gilles | Faye Jean-Charles
Volume: 8
Issue: 1
Year: 2008
Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins

Author(s): Kim Yoon-Sik | Lee Yong-Hwa | Kim Hyun-Soon | Kim Mi-Sun | Hahn Kyu-Woong | Ko Jeong-Heon | Joung Hyouk | Jeon Jae-Heung
Volume: 8
Issue: 1
Year: 2008
Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers

Author(s): Hsu Charlie | Uludağ Hasan
Volume: 8
Issue: 1
Year: 2008
Simplified approaches for the development of an ELISA to detect circulating autoantibodies to p53 in cancer patients

Author(s): Cressey Ratchada | Pimpa Saranya | Chewaskulyong Busyamas | Lertprasertsuke Nirush | Saeteng Somchareon | Tayapiwatana Chatchai | Kasinrerk Watchara
Volume: 8
Issue: 1
Year: 2008
Effect of ionic strength and presence of serum on lipoplexes structure monitorized by FRET

Author(s): Madeira Catarina | Loura Luís | Prieto Manuel | Fedorov Aleksander | Aires-Barros M Raquel
Volume: 8
Issue: 1
Year: 2008
Design of a zinc finger protein binding a sequence upstream of the A20 gene

Author(s): Wei Yong | Ying Dajun | Hou Chunli | Cui Xiaoping | Zhu Chuhong
Volume: 8
Issue: 1
Year: 2008
Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coli protein, RpoS

Author(s): Park Jin-Seung | Han Kyung-Yeon | Lee Jong-Ho | Song Jong-Am | Ahn Keum-Young | Seo Hyuk-Seong | Sim Sang-Jun | Kim Seung-Wook | Lee Jeewon
Volume: 8
Issue: 1
Year: 2008
Production of functionally active Penicillium chrysogenum isopenicillin N synthase in the yeast Hansenula polymorpha

Author(s): Gidijala Loknath | Bovenberg Roel | Klaassen Paul | van der Klei Ida | Veenhuis Marten | Kiel Jan
Volume: 8
Issue: 1
Year: 2008
Spherezymes: A novel structured self-immobilisation enzyme technology

Author(s): Brady Dean | Jordaan Justin | Simpson Clinton | Chetty Avashnee | Arumugam Cherise | Moolman Francis
Volume: 8
Issue: 1
Year: 2008
Enhanced gene expression from retroviral vectors

Author(s): Blø Magnus | Micklem David | Lorens James
Volume: 8
Issue: 1
Year: 2008
Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues

Author(s): Frumkin Dan | Wasserstrom Adam | Itzkovitz Shalev | Harmelin Alon | Rechavi Gideon | Shapiro Ehud
Volume: 8
Issue: 1
Year: 2008
Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface

Author(s): Jiang Zhengbing | Gao Bei | Ren Ren | Tao Xingyi | Ma Yushu | Wei Dongzhi
Volume: 8
Issue: 1
Year: 2008
Protein kinase substrate identification on functional protein arrays

Author(s): Meng Lihao | Michaud Gregory | Merkel Janie | Zhou Fang | Huang Jing | Mattoon Dawn | Schweitzer Barry
Volume: 8
Issue: 1
Year: 2008
Negative selection of chronic lymphocytic leukaemia cells using a bifunctional rosette-based antibody cocktail

Author(s): Essakali Salim | Carney Dennis | Westerman David | Gambell Peter | Seymour John | Dobrovic Alexander
Volume: 8
Issue: 1
Year: 2008
Enhancement of hybridoma formation, clonability and cell proliferation in a nanoparticle-doped aqueous environment

Author(s): Gavrilov-Yusim Natalie | Hahiashvili Ekaterina | Tashker Marina | Yavelsky Victoria | Karnieli Ohad | Lobel Leslie
Volume: 8
Issue: 1
Year: 2008
Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations

Author(s): Wegerer Angelika | Sun Tianqi | Altenbuchner Josef
Volume: 8
Issue: 1
Year: 2008
Quantitative comparison of DNA detection by GFP-lac repressor tagging, fluorescence in situ hybridization and immunostaining

Author(s): Kim Il-Han | Nagel Jens | Otten Simone | Knerr Boris | Eils Roland | Rohr Karl | Dietzel Steffen
Volume: 7
Issue: 1
Year: 2007
Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells

Author(s): Tragoolpua Khajornsak | Intasai Nutjeera | Kasinrerk Watchara | Mai Sabine | Yuan Yuan | Tayapiwatana Chatchai
Volume: 8
Issue: 1
Year: 2008
High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

Author(s): Chang Hsueh-Wei | Cheng Chun-An | Gu De-Leung | Chang Chia-Che | Su San-Hua | Wen Cheng-Hao | Chou Yii-Cheng | Chou Ta-Ching | Yao Cheng-Te | Tsai Chi-Li | Cheng Chien-Chung
Volume: 8
Issue: 1
Year: 2008
Improved RNA quality and TaqMan® Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials

Author(s): Li Jinghuan | Smyth Paul | Cahill Susanne | Denning Karen | Flavin Richard | Aherne Sinead | Pirotta Marco | Guenther Simone | O'Leary John | Sheils Orla
Volume: 8
Issue: 1
Year: 2008
Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection

Author(s): Schiedner Gudrun | Hertel Sabine | Bialek Corinna | Kewes Helmut | Waschütza Gero | Volpers Christoph
Volume: 8
Issue: 1
Year: 2008
Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord

Author(s): Ronsyn Mark | Daans Jasmijn | Spaepen Gie | Chatterjee Shyama | Vermeulen Katrien | D'Haese Patrick | Van Tendeloo Viggo | Van Marck Eric | Ysebaert Dirk | Berneman Zwi | Jorens Philippe | Ponsaerts Peter
Volume: 7
Issue: 1
Year: 2007
Self-assembling Fmoc dipeptide hydrogel for in situ 3D cell culturing

Author(s): Liebmann Thomas | Rydholm Susanna | Akpe Victor | Brismar Hjalmar
Volume: 7
Issue: 1
Year: 2007
Comparison of chicken 7SK and U6 RNA polymerase III promoters for short hairpin RNA expression

Author(s): Bannister Stephanie | Wise Terry | Cahill David | Doran Timothy
Volume: 7
Issue: 1
Year: 2007
Expression of HPV-11 L1 protein in transgenic Arabidopsis thaliana and Nicotiana tabacum

Author(s): Kohl Thomas | Hitzeroth Inga | Christensen Neil | Rybicki Edward
Volume: 7
Issue: 1
Year: 2007
A new generation of pPRIG-based retroviral vectors

Author(s): Albagli-Curiel Olivier | Lécluse Yann | Pognonec Philippe | Boulukos Kim | Martin Patrick
Volume: 7
Issue: 1
Year: 2007
Improved functional expression of recombinant human ether-a-go-go (hERG) K+ channels by cultivation at reduced temperature

Author(s): Chen Mao | Sandow Shaun | Doceul Virginie | Chen Yu | Harper Heather | Hamilton Bruce | Meadows Helen | Trezise Derek | Clare Jeff
Volume: 7
Issue: 1
Year: 2007
A focused antibody library for selecting scFvs expressed at high levels in the cytoplasm

Author(s): Philibert Pascal | Stoessel Audrey | Wang Wei | Sibler Annie-Paule | Bec Nicole | Larroque Christian | Saven Jeffery | Courtête Jérôme | Weiss Etienne | Martineau Pierre
Volume: 7
Issue: 1
Year: 2007
Single-cell duplex RT-LATE-PCR reveals Oct4 and Xist RNA gradients in 8-cell embryos

Author(s): Hartshorn Cristina | Eckert Judith | Hartung Odelya | Wangh Lawrence
Volume: 7
Issue: 1
Year: 2007
Compatible solutes from hyperthermophiles improve the quality of DNA microarrays

Author(s): Mascellani Nicoletta | Liu Xiuping | Rossi Simona | Marchesini Jlenia | Valentini Davide | Arcelli Diego | Taccioli Cristian | Helmer Citterich Mauro | Liu Chang-Gong | Evangelisti Rita | Russo Giandomenico | Santos Jorge | Croce Carlo | Volinia Stefano
Volume: 7
Issue: 1
Year: 2007
Adeno-associated viral vectors engineered for macrolide-adjustable transgene expression In mammalian cells and mice

Author(s): Fluri David | Baba Marie | Fussenegger Martin
Volume: 7
Issue: 1
Year: 2007
Comparison of estimation capabilities of response surface methodology (RSM) with artificial neural network (ANN) in lipase-catalyzed synthesis of palm-based wax ester

Author(s): Basri Mahiran | Rahman Raja | Ebrahimpour Afshin | Salleh Abu | Gunawan Erin | Rahman Mohd
Volume: 7
Issue: 1
Year: 2007
Isolation of anti-toxin single domain antibodies from a semi-synthetic spiny dogfish shark display library

Author(s): Liu Jinny | Anderson George | Goldman Ellen
Volume: 7
Issue: 1
Year: 2007
Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications

Author(s): Mao Fei | Leung Wai-Yee | Xin Xing
Volume: 7
Issue: 1
Year: 2007
Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a new strategy for generating fully non-fucosylated therapeutic antibodies with enhanced ADCC

Author(s): Imai-Nishiya Harue | Mori Katsuhiro | Inoue Miho | Wakitani Masako | Iida Shigeru | Shitara Kenya | Satoh Mitsuo
Volume: 7
Issue: 1
Year: 2007
Tumor-infiltrating B lymphocytes as an efficient source of highly specific immunoglobulins recognizing tumor cells

Author(s): Pavoni Emiliano | Monteriù Giorgia | Santapaola Daniela | Petronzelli Fiorella | Anastasi Anna | Pelliccia Angela | D'Alessio Valeria | De Santis Rita | Minenkova Olga
Volume: 7
Issue: 1
Year: 2007
Long term expression of bicistronic vector driven by the FGF-1 IRES in mouse muscle

Author(s): Allera-Moreau Camille | Delluc-Clavières Aurélie | Castano Caroline | Van den Berghe Loïc | Golzio Muriel | Moreau Marc | Teissié Justin | Arnal Jean-François | Prats Anne-Catherine
Volume: 7
Issue: 1
Year: 2007
An integrative expression vector for Actinosynnema pretiosum

Author(s): Goh Shan | Camattari Andrea | Ng Daniel | Song Ruth | Madden Kevin | Westpheling Janet | Wong Victor
Volume: 7
Issue: 1
Year: 2007
Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization

Author(s): Katashkina Joanna | Kuvaeva Tatiana | Andreeva Irina | Skorokhodova Alexandra | Biryukova Irina | Tokmakova Irina | Golubeva Lubov | Mashko Sergey
Volume: 7
Issue: 1
Year: 2007
Revealing biases inherent in recombination protocols

Author(s): Chaparro-Riggers Javier | Loo Bernard | Polizzi Karen | Gibbs Phillip | Tang Xiao-Song | Nelson Mark | Bommarius Andreas
Volume: 7
Issue: 1
Year: 2007
In situ detection of non-polyadenylated RNA molecules using Turtle Probes and target primed rolling circle PRINS

Author(s): Stougaard Magnus | Lohmann Jakob | Zajac Magdalena | Hamilton-Dutoit Stephen | Koch Jørn
Volume: 7
Issue: 1
Year: 2007
High content image analysis for human H4 neuroglioma cells exposed to CuO nanoparticles

Author(s): Li Fuhai | Zhou Xiaobo | Zhu Jinmin | Ma Jinwen | Huang Xudong | Wong Stephen
Volume: 7
Issue: 1
Year: 2007
Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homolog

Author(s): Jaluria Pratik | Betenbaugh Michael | Konstantopoulos Konstantinos | Shiloach Joseph
Volume: 7
Issue: 1
Year: 2007
Trinucleotide cassettes increase diversity of T7 phage-displayed peptide library

Author(s): Krumpe Lauren | Schumacher Kathryn | McMahon James | Makowski Lee | Mori Toshiyuki
Volume: 7
Issue: 1
Year: 2007
Induction of protective immunity in swine by recombinant bamboo mosaic virus expressing foot-and-mouth disease virus epitopes

Author(s): Yang Chung-Da | Liao Jia-Teh | Lai Chen-Yen | Jong Ming-Hwa | Liang Chi-Ming | Lin Yeou-Liang | Lin Na-Sheng | Hsu Yau-Heiu | Liang Shu-Mei
Volume: 7
Issue: 1
Year: 2007
A systematic approach for testing expression of human full-length proteins in cell-free expression systems

Author(s): Langlais Claudia | Guilleaume Birgit | Wermke Nadja | Scheuermann Tina | Ebert Lars | LaBaer Joshua | Korn Bernhard
Volume: 7
Issue: 1
Year: 2007
Characterization of the effect of sample quality on high density oligonucleotide microarray data using progressively degraded rat liver RNA

Author(s): Thompson Karol | Pine P Scott | Rosenzweig Barry | Turpaz Yaron | Retief Jacques
Volume: 7
Issue: 1
Year: 2007
Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome

Author(s): Cox Mandy | Layton Sherryll | Jiang Tieshan | Cole Kim | Hargis Billy | Berghman Luc | Bottje Walter | Kwon Young
Volume: 7
Issue: 1
Year: 2007
New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase

Author(s): Cecchini Davide | Serra Immacolata | Ubiali Daniela | Terreni Marco | Albertini Alessandra
Volume: 7
Issue: 1
Year: 2007
A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods

Author(s): Chung Bong Geun | Park Jeong Won | Hu Jia | Huang Carlos | Monuki Edwin | Jeon Noo Li
Volume: 7
Issue: 1
Year: 2007
Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilactici UVA1

Author(s): Mathys Sophie | von Ah Ueli | Lacroix Christophe | Staub Ernö | Mini Raffaella | Cereghetti Tania | Meile Leo
Volume: 7
Issue: 1
Year: 2007
High performance microbiological transformation of L-tyrosine to L-dopa by Yarrowia lipolytica NRRL-143

Author(s): Ali Sikander | Shultz Jeffry | Ikram-ul-Haq
Volume: 7
Issue: 1
Year: 2007
One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX

Author(s): Nitsche Andreas | Kurth Andreas | Dunkhorst Anna | Pänke Oliver | Sielaff Hendrik | Junge Wolfgang | Muth Doreen | Scheller Frieder | Stöcklein Walter | Dahmen Claudia | Pauli Georg | Kage Andreas
Volume: 7
Issue: 1
Year: 2007
A minimal peach type II chlorophyll a/b-binding protein promoter retains tissue-specificity and light regulation in tomato

Author(s): Bassett Carole | Callahan Ann | Artlip Timothy | Scorza Ralph | Srinivasan Chinnathambi
Volume: 7
Issue: 1
Year: 2007
Laser-scanning velocimetry: A confocal microscopy method for quantitative measurement of cardiovascular performance in zebrafish embryos and larvae

Author(s): Malone Michael | Sciaky Noah | Stalheim Lisa | Hahn Klaus | Linney Elwood | Johnson Gary
Volume: 7
Issue: 1
Year: 2007
Lentiviral vectors encoding tetracycline-dependent repressors and transactivators for reversible knockdown of gene expression: a comparative study

Author(s): Pluta Krzysztof | Diehl William | Zhang Xian-Yang | Kutner Robert | Bialkowska Agnieszka | Reiser Jakob
Volume: 7
Issue: 1
Year: 2007
Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models

Author(s): Di Niro Roberto | Ziller Federica | Florian Fiorella | Crovella Sergio | Stebel Marco | Bestagno Marco | Burrone Oscar | Bradbury Andrew | Secco Paola | Marzari Roberto | Sblattero Daniele
Volume: 7
Issue: 1
Year: 2007
Knockdown of the bovine prion gene PRNP by RNA interference (RNAi) technology

Author(s): Sutou Shizuyo | Kunishi Miho | Kudo Toshiyuki | Wongsrikeao Pimprapar | Miyagishi Makoto | Otoi Takeshige
Volume: 7
Issue: 1
Year: 2007
A novel real-time ultrasonic method for prion protein detection using plasminogen as a capture molecule

Author(s): Negredo Carmen | Monks Eoin | Sweeney Torres
Volume: 7
Issue: 1
Year: 2007
Enzymatic engineering of the porcine genome with transposons and recombinases

Author(s): Clark Karl | Carlson Daniel | Foster Linda | Kong Byung-Whi | Foster Douglas | Fahrenkrug Scott
Volume: 7
Issue: 1
Year: 2007
Domain selection combined with improved cloning strategy for high throughput expression of higher eukaryotic proteins

Author(s): Chen Yunjia | Qiu Shihong | Luan Chi-Hao | Luo Ming
Volume: 7
Issue: 1
Year: 2007
Cloning and characterization of an adenoviral vector for highly efficient and doxycycline – suppressible expression of bioactive human single – chain interleukin 12 in colon cancer

Author(s): Wulff Holger | Krieger Thorsten | Krüger Karen | Stahmer Ingrid | Thaiss Friedrich | Schäfer Hansjörg | Block Andreas
Volume: 7
Issue: 1
Year: 2007
Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells

Author(s): Li Jinghuan | Smyth Paul | Flavin Richard | Cahill Susanne | Denning Karen | Aherne Sinead | Guenther Simone | O'Leary John | Sheils Orla
Volume: 7
Issue: 1
Year: 2007
Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering

Author(s): De Mey Marjan | Maertens Jo | Lequeux Gaspard | Soetaert Wim | Vandamme Erick
Volume: 7
Issue: 1
Year: 2007
Single fluorescent protein-based Ca2+ sensors with increased dynamic range

Author(s): Souslova Ekaterina | Belousov Vsevolod | Lock John | Strömblad Staffan | Kasparov Sergey | Bolshakov Alexey | Pinelis Vsevolod | Labas Yulii | Lukyanov Sergey | Mayr Lorenz | Chudakov Dmitriy
Volume: 7
Issue: 1
Year: 2007
Generation of human scFvs antibodies recognizing a prion protein epitope expressed on the surface of human lymphoblastoid cells

Author(s): Flego Michela | Ascione Alessandro | Zamboni Silvia | Dupuis Maria | Imperiale Valentina | Cianfriglia Maurizio
Volume: 7
Issue: 1
Year: 2007
Recombinant nucleases CEL I from celery and SP I from spinach for mutation detection

Author(s): Pimkin Maxim | Caretti Elena | Canutescu Adrian | Yeung Jeffrey | Cohn Heather | Chen Yibai | Oleykowski Catherine | Bellacosa Alfonso | Yeung Anthony
Volume: 7
Issue: 1
Year: 2007
Marker tolerant, immunocompetent animals as a new tool for regenerative medicine and long-term cell tracking

Author(s): Odörfer Kathrin | Unger Nina | Weber Karin | Sandgren Eric | Erben Reinhold
Volume: 7
Issue: 1
Year: 2007
Physiological evaluation of the filamentous fungus Trichoderma reesei in production processes by marker gene expression analysis

Author(s): Rautio Jari | Bailey Michael | Kivioja Teemu | Söderlund Hans | Penttilä Merja | Saloheimo Markku
Volume: 7
Issue: 1
Year: 2007
Isolation of mesenchymal stem cells from equine umbilical cord blood

Author(s): Koch Thomas | Heerkens Tammy | Thomsen Preben | Betts Dean
Volume: 7
Issue: 1
Year: 2007
Inhibition of hydrogen uptake in Escherichia coli by expressing the hydrogenase from the cyanobacterium Synechocystis sp. PCC 6803

Author(s): Maeda Toshinari | Vardar Gönül | Self William | Wood Thomas
Volume: 7
Issue: 1
Year: 2007
Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor

Author(s): Dmytruk Kostyantyn | Smutok Oleh | Ryabova Olena | Gayda Galyna | Sibirny Volodymyr | Schuhmann Wolfgang | Gonchar Mykhailo | Sibirny Andriy
Volume: 7
Issue: 1
Year: 2007
Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli

Author(s): de Marco Ario | Deuerling Elke | Mogk Axel | Tomoyasu Toshifumi | Bukau Bernd
Volume: 7
Issue: 1
Year: 2007
Potential pitfalls in the accuracy of analysis of natural sense-antisense RNA pairs by reverse transcription-PCR

Author(s): Haddad Fadia | Qin Anqi | Giger Julie | Guo Hongyan | Baldwin Kenneth
Volume: 7
Issue: 1
Year: 2007
Improvement of activity and stability of chloroperoxidase by chemical modification

Author(s): Liu Jian-Zhong | Wang Min
Volume: 7
Issue: 1
Year: 2007
Engineering proteinase K using machine learning and synthetic genes

Author(s): Liao Jun | Warmuth Manfred | Govindarajan Sridhar | Ness Jon | Wang Rebecca | Gustafsson Claes | Minshull Jeremy
Volume: 7
Issue: 1
Year: 2007
High level expression of soluble glycoproteins in the allantoic fluid of embryonated chicken eggs using a Sendai virus minigenome system

Author(s): Corral Teresa | Ver Lorena | Mottet Geneviève | Cano Olga | García-Barreno Blanca | Calder Lesley | Skehel John | Roux Laurent | Melero José
Volume: 7
Issue: 1
Year: 2007
Mechanism of chimera formation during the Multiple Displacement Amplification reaction

Author(s): Lasken Roger | Stockwell Timothy
Volume: 7
Issue: 1
Year: 2007
RNA mutagenesis yields highly diverse mRNA libraries for in vitro protein evolution

Author(s): Kopsidas George | Carman Rachael | Stutt Emma | Raicevic Anna | Roberts Anthony | Siomos Mary-Anne | Dobric Nada | Pontes-Braz Luisa | Coia Greg
Volume: 7
Issue: 1
Year: 2007
Development of a one-step embryonic stem cell-based assay for the screening of sprouting angiogenesis

Author(s): Hermant Bastien | Desroches-Castan Agnès | Dubessay Marie-Laure | Prandini Marie-Hélène | Huber Philippe | Vittet Daniel
Volume: 7
Issue: 1
Year: 2007
Effective inhibition of lytic development of bacteriophages λ, P1 and T4 by starvation of their host, Escherichia coli

Author(s): Łoś Marcin | Golec Piotr | Łoś Joanna | Węglewska-Jurkiewicz Anna | Czyż Agata | Węgrzyn Alicja | Węgrzyn Grzegorz | Neubauer Peter
Volume: 7
Issue: 1
Year: 2007
Single chain Fab (scFab) fragment

Author(s): Hust Michael | Jostock Thomas | Menzel Christian | Voedisch Bernd | Mohr Anja | Brenneis Mariam | Kirsch Martina | Meier Doris | Dübel Stefan
Volume: 7
Issue: 1
Year: 2007
High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol

Author(s): Papagianni Maria | Avramidis Nicholaos | Filioussis George
Volume: 7
Issue: 1
Year: 2007
An adenovirus prime/plasmid boost strategy for induction of equipotent immune responses to two dengue virus serotypes

Author(s): Khanam Saima | Rajendra Pilankatta | Khanna Navin | Swaminathan Sathyamangalam
Volume: 7
Issue: 1
Year: 2007
Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells

Author(s): Marusic Carla | Nuttall James | Buriani Giampaolo | Lico Chiara | Lombardi Raffaele | Baschieri Selene | Benvenuto Eugenio | Frigerio Lorenzo
Volume: 7
Issue: 1
Year: 2007
Novel system uses probasin-based promoter, transcriptional silencers and amplification loop to induce high-level prostate expression

Author(s): Woraratanadharm Jan | Rubinchik Semyon | Yu Hong | Dong John
Volume: 7
Issue: 1
Year: 2007
Production of transgenic strawberries by temporary immersion bioreactor system and verification by TAIL-PCR

Author(s): Hanhineva Kati | Kärenlampi Sirpa
Volume: 7
Issue: 1
Year: 2007
Plastoglobules: a new address for targeting recombinant proteins in the chloroplast

Author(s): Vidi Pierre-Alexandre | Kessler Felix | Bréhélin Claire
Volume: 7
Issue: 1
Year: 2007
Improved single-chain transactivators of the Tet-On gene expression system

Author(s): Zhou Xue | Symons Jori | Hoppes Rieuwert | Krueger Christel | Berens Christian | Hillen Wolfgang | Berkhout Ben | Das Atze
Volume: 7
Issue: 1
Year: 2007
Elimination of laboratory ozone leads to a dramatic improvement in the reproducibility of microarray gene expression measurements

Author(s): Branham William | Melvin Cathy | Han Tao | Desai Varsha | Moland Carrie | Scully Adam | Fuscoe James
Volume: 7
Issue: 1
Year: 2007
Development and validation of vectors containing multiple siRNA expression cassettes for maximizing the efficiency of gene silencing

Author(s): Wang Shunqing | Shi Zhenqi | Liu Wei | Jules Joel | Feng Xu
Volume: 6
Issue: 1
Year: 2006
Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBac

Author(s): Shi Xianzong | Harrison Robert | Hollister Jason | Mohammed Ahmed | Fraser Malcolm | Jarvis Donald
Volume: 7
Issue: 1
Year: 2007
Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains

Author(s): Olichon Aurelien | Schweizer Daniel | Muyldermans Serge | de Marco Ario
Volume: 7
Issue: 1
Year: 2007
A time- and dose-dependent STAT1 expression system

Author(s): Leitner Nicole | Strobl Birgit | Bokor Marion | Painz Ronald | Kolbe Thomas | Rülicke Thomas | Müller Mathias | Karaghiosoff Marina
Volume: 6
Issue: 1
Year: 2006
Internalization of novel non-viral vector TAT-streptavidin into human cells

Author(s): Rinne Johanna | Albarran Brian | Jylhävä Juulia | Ihalainen Teemu | Kankaanpää Pasi | Hytönen Vesa | Stayton Patrick | Kulomaa Markku | Vihinen-Ranta Maija
Volume: 7
Issue: 1
Year: 2007
On the activity loss of hydrolases in organic solvents: II. a mechanistic study of subtilisin Carlsberg

Author(s): Castillo Betzaida | Bansal Vibha | Ganesan Ashok | Halling Peter | Secundo Francesco | Ferrer Amaris | Griebenow Kai | Barletta Gabriel
Volume: 6
Issue: 1
Year: 2006
Multiplexed expression and screening for recombinant protein production in mammalian cells

Author(s): Chapple Susan | Crofts Anna | Shadbolt S Paul | McCafferty John | Dyson Michael
Volume: 6
Issue: 1
Year: 2006
Generation and characterization of a stable cell population releasing fluorescent HIV-1-based Virus Like Particles in an inducible way

Author(s): Muratori Claudia | D'Aloja Paola | Superti Fabiana | Tinari Antonella | Sol-Foulon Nathalie | Sparacio Sandra | Bosch Valerie | Schwartz Olivier | Federico Maurizio
Volume: 6
Issue: 1
Year: 2006
Two-temperature LATE-PCR endpoint genotyping

Author(s): Sanchez J Aquiles | Abramowitz Jessica | Salk Jesse | Reis Arthur | Rice John | Pierce Kenneth | Wangh Lawrence
Volume: 6
Issue: 1
Year: 2006
In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes

Author(s): Bestoso Federica | Ottaggio Laura | Armirotti Andrea | Balbi Alessandro | Damonte Gianluca | Degan Paolo | Mazzei Mauro | Cavalli Francesca | Ledda Bernardetta | Miele Mariangela
Volume: 6
Issue: 1
Year: 2006
A simple vector system to improve performance and utilisation of recombinant antibodies

Author(s): Martin Cecile | Rojas Gertrudis | Mitchell Joanne | Vincent Karen | Wu Jiahua | McCafferty John | Schofield Darren
Volume: 6
Issue: 1
Year: 2006
Target labelling for the detection and profiling of microRNAs expressed in CNS tissue using microarrays

Author(s): Saba Reuben | Booth Stephanie
Volume: 6
Issue: 1
Year: 2006
Transformation of Anaplasma phagocytophilum

Author(s): Felsheim Roderick | Herron Michael | Nelson Curtis | Burkhardt Nicole | Barbet Anthony | Kurtti Timothy | Munderloh Ulrike
Volume: 6
Issue: 1
Year: 2006
Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A

Author(s): Erkens Tim | Van Poucke Mario | Vandesompele Jo | Goossens Karen | Van Zeveren Alex | Peelman Luc
Volume: 6
Issue: 1
Year: 2006
Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon

Author(s): Geurts Aron | Wilber Andrew | Carlson Corey | Lobitz Paul | Clark Karl | Hackett Perry | McIvor R Scott | Largaespada David
Volume: 6
Issue: 1
Year: 2006
Routes to improving the reliability of low level DNA analysis using real-time PCR

Author(s): Ellison Stephen | English Claire | Burns Malcolm | Keer Jacquie
Volume: 6
Issue: 1
Year: 2006
Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms

Author(s): Cankar Katarina | Štebih Dejan | Dreo Tanja | Žel Jana | Gruden Kristina
Volume: 6
Issue: 1
Year: 2006
Development of a Premature Stop Codon-detection method based on a bacterial two-hybrid system

Author(s): Real Sebastián | Marzese Diego | Gomez Laura | Mayorga Luis | Roqué María
Volume: 6
Issue: 1
Year: 2006
Insulated piggyBac vectors for insect transgenesis

Author(s): Sarkar Abhimanyu | Atapattu Asela | Belikoff Esther | Heinrich Jörg | Li Xuelei | Horn Carsten | Wimmer Ernst | Scott Maxwell
Volume: 6
Issue: 1
Year: 2006
Generation of non-genomic oligonucleotide tag sequences for RNA template-specific PCR

Author(s): Pinto Fernando | Svensson Håkan | Lindblad Peter
Volume: 6
Issue: 1
Year: 2006
PCR-based generation of shRNA libraries from cDNAs

Author(s): Du Cheng | Ge Baosheng | Liu Zhongfeng | Fu Kai | Chan Wing | McKeithan Timothy
Volume: 6
Issue: 1
Year: 2006
In planta production of two peptides of the Classical Swine Fever Virus (CSFV) E2 glycoprotein fused to the coat protein of potato virus X

Author(s): Marconi Gianpiero | Albertini Emidio | Barone Pierluigi | De Marchis Francesca | Lico Chiara | Marusic Carla | Rutili Domenico | Veronesi Fabio | Porceddu Andrea
Volume: 6
Issue: 1
Year: 2006
pTcINDEX: a stable tetracycline-regulated expression vector for Trypanosoma cruzi

Author(s): Taylor Martin | Kelly John
Volume: 6
Issue: 1
Year: 2006
Reversible inactivation of the transcriptional function of P53 protein by farnesylation

Author(s): Couderc Bettina | Penary Marie | Tohfe Mustapha | Pradines Anne | Casteignau Antoine | Berg Danièle | Favre Gilles
Volume: 6
Issue: 1
Year: 2006
Construction, purification, and characterization of a chimeric TH1 antagonist

Author(s): Bello-Rivero Iraldo | Torrez-Ruiz Yeny | Blanco-Garcés Elizabeth | Pentón-Rol Giselle | Fernández-Batista Osmani | Javier-González Luís | Gerónimo-Perez Haydee | López-Saura Pedro
Volume: 6
Issue: 1
Year: 2006
Selection of a set of reliable reference genes for quantitative real-time PCR in normal equine skin and in equine sarcoids

Author(s): Bogaert Lies | Van Poucke Mario | De Baere Cindy | Peelman Luc | Gasthuys Frank | Martens Ann
Volume: 6
Issue: 1
Year: 2006
Optical imaging of luminescence for in vivo quantification of gene electrotransfer in mouse muscle and knee

Author(s): Bloquel C | Trollet C | Pradines E | Seguin J | Scherman D | Bureau MF
Volume: 6
Issue: 1
Year: 2006
Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli

Author(s): Watts Kevin | Lee Pyung | Schmidt-Dannert Claudia
Volume: 6
Issue: 1
Year: 2006
RecET driven chromosomal gene targeting to generate a RecA deficient Escherichia coli strain for Cre mediated production of minicircle DNA

Author(s): Tolmachov Oleg | Palaszewski Iwona | Bigger Brian | Coutelle Charles
Volume: 6
Issue: 1
Year: 2006
Modification of the Creator recombination system for proteomics applications – improved expression by addition of splice sites

Author(s): Colwill Karen | Wells Clark | Elder Kelly | Goudreault Marilyn | Hersi Kadija | Kulkarni Sarang | Hardy W Rod | Pawson Tony | Morin Gregg
Volume: 6
Issue: 1
Year: 2006
A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production

Author(s): Cabrita Lisa | Dai Weiwen | Bottomley Stephen
Volume: 6
Issue: 1
Year: 2006
Criteria for effective design, construction, and gene knockdown by shRNA vectors

Author(s): Taxman Debra | Livingstone Laura | Zhang Jinghua | Conti Brian | Iocca Heather | Williams Kristi | Lich John | Ting Jenny | Reed William
Volume: 6
Issue: 1
Year: 2006
Strict control of telomerase activation using Cre-mediated inversion

Author(s): Ungrin Mark | Harrington Lea
Volume: 6
Issue: 1
Year: 2006
A novel expression system of domain I of human beta2 glycoprotein I in Escherichia coli

Author(s): Ioannou Yiannis | Giles Ian | Lambrianides Anastasia | Richardson Chris | Pearl Laurence | Latchman David | Isenberg David | Rahman Anisur
Volume: 6
Issue: 1
Year: 2006
Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals

Author(s): Gendron Daniel | Carriero Sandra | Garneau Daniel | Villemaire Jonathan | Klinck Roscoe | Elela Sherif | Damha Masad | Chabot Benoit
Volume: 6
Issue: 1
Year: 2006
Sequence specific visual detection of LAMP reactions by addition of cationic polymers

Author(s): Mori Yasuyoshi | Hirano Tsuyoshi | Notomi Tsugunori
Volume: 6
Issue: 1
Year: 2006
Development of a new bicistronic retroviral vector with strong IRES activity

Author(s): Martin Patrick | Albagli Olivier | Poggi Marie | Boulukos Kim | Pognonec Philippe
Volume: 6
Issue: 1
Year: 2006
Pore forming polyalkylpyridinium salts from marine sponges versus synthetic lipofection systems: distinct tools for intracellular delivery of cDNA and siRNA

Author(s): McLaggan Debra | Adjimatera Noppadon | Sepčić Kristina | Jaspars Marcel | MacEwan David | Blagbrough Ian | Scott Roderick
Volume: 6
Issue: 1
Year: 2006
Open field trial of genetically modified parthenocarpic tomato: seedlessness and fruit quality

Author(s): Rotino Giuseppe | Acciarri Nazareno | Sabatini Emidio | Mennella Giuseppe | Lo Scalzo Roberto | Maestrelli Andrea | Molesini Barbara | Pandolfini Tiziana | Scalzo Jessica | Mezzetti Bruno | Spena Angelo
Volume: 5
Issue: 1
Year: 2005
Genetic transformation of novel isolates of chicken Lactobacillus bearing probiotic features for expression of heterologous proteins: a tool to develop live oral vaccines

Author(s): Mota Rodrigo | Moreira João | Souza Marcelo | Fátima Horta M | Teixeira Santuza | Neumann Elisabeth | Nicoli Jacques | Nunes Álvaro
Volume: 6
Issue: 1
Year: 2006
Design and cloning strategies for constructing shRNA expression vectors

Author(s): McIntyre Glen | Fanning Gregory
Volume: 6
Issue: 1
Year: 2006
Use of endogenous signal sequences for transient production and efficient secretion by moss (Physcomitrella patens) cells

Author(s): Schaaf Andreas | Tintelnot Stefanie | Baur Armin | Reski Ralf | Gorr Gilbert | Decker Eva
Volume: 5
Issue: 1
Year: 2005
Avidin related protein 2 shows unique structural and functional features among the avidin protein family

Author(s): Hytönen Vesa | Määttä Juha | Kidron Heidi | Halling Katrin | Hörhä Jarno | Kulomaa Tuomas | Nyholm Thomas | Johnson Mark | Salminen Tiina | Kulomaa Markku | Airenne Tomi
Volume: 5
Issue: 1
Year: 2005
Nymphal RNAi: systemic RNAi mediated gene knockdown in juvenile grasshopper

Author(s): Dong Ying | Friedrich Markus
Volume: 5
Issue: 1
Year: 2005
Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine spongiform encephalopathy

Author(s): Trieschmann Lothar | Santos Alexander | Kaschig Katja | Torkler Sandra | Maas Elke | Schätzl Hermann | Böhm Gerald
Volume: 5
Issue: 1
Year: 2005
Effects of DNA mass on multiple displacement whole genome amplification and genotyping performance

Author(s): Bergen Andrew | Qi Ying | Haque Kashif | Welch Robert | Chanock Stephen
Volume: 5
Issue: 1
Year: 2005
Genetic and spectrally distinct in vivo imaging: embryonic stem cells and mice with widespread expression of a monomeric red fluorescent protein

Author(s): Long Jonathan | Lackan Chantal | Hadjantonakis Anna-Katerina
Volume: 5
Issue: 1
Year: 2005
Efficient assembly of de novo human artificial chromosomes from large genomic loci

Author(s): Basu Joydeep | Compitello George | Stromberg Gregory | Willard Huntington | Van Bokkelen Gil
Volume: 5
Issue: 1
Year: 2005
Viral promoters can initiate expression of toxin genes introduced into Escherichia coli

Author(s): Lewin Astrid | Mayer Martin | Chusainow Janet | Jacob Daniela | Appel Bernd
Volume: 5
Issue: 1
Year: 2005
Cell membrane array fabrication and assay technology

Author(s): Yamazaki Victoria | Sirenko Oksana | Schafer Robert | Nguyen Luat | Gutsmann Thomas | Brade Lore | Groves Jay
Volume: 5
Issue: 1
Year: 2005
CpG-island fragments from the HNRPA2B1/CBX3 genomic locus reduce silencing and enhance transgene expression from the hCMV promoter/enhancer in mammalian cells

Author(s): Williams Steven | Mustoe Tracey | Mulcahy Tony | Griffiths Mark | Simpson David | Antoniou Michael | Irvine Alistair | Mountain Andrew | Crombie Robert
Volume: 5
Issue: 1
Year: 2005
An inter-platform repeatability study investigating real-time amplification of plasmid DNA

Author(s): Donald Carol | Qureshi Fizza | Burns Malcolm | Holden Marcia | Blasic Joseph | Woolford Alison
Volume: 5
Issue: 1
Year: 2005
High-throughput kinase assays with protein substrates using fluorescent polymer superquenching

Author(s): Rininsland Frauke | Stankewicz Casey | Weatherford Wendy | McBranch Duncan
Volume: 5
Issue: 1
Year: 2005
Characterisation and application of a bovine U6 promoter for expression of short hairpin RNAs

Author(s): Lambeth Luke | Moore Robert | Muralitharan Morley | Dalrymple Brian | McWilliam Sean | Doran Timothy
Volume: 5
Issue: 1
Year: 2005
A simple technique for quantifying apoptosis in 96-well plates

Author(s): Ribble Deborah | Goldstein Nathaniel | Norris David | Shellman Yiqun
Volume: 5
Issue: 1
Year: 2005
Low density DNA microarray for detection of most frequent TP53 missense point mutations

Author(s): Rangel-López Angélica | Maldonado-Rodríguez Rogelio | Salcedo-Vargas Mauricio | Espinosa-Lara Juana | Méndez-Tenorio Alfonso | Beattie Kenneth
Volume: 5
Issue: 1
Year: 2005
Assembly of a gene sequence tag microarray by reversible biotin-streptavidin capture for transcript analysis of Arabidopsis thaliana

Author(s): Wirta Valtteri | Holmberg Anders | Lukacs Morten | Nilsson Peter | Hilson Pierre | Uhlén Mathias | Bhalerao Rishikesh | Lundeberg Joakim
Volume: 5
Issue: 1
Year: 2005
Functional complementation of RNA interference mutants in trypanosomes

Author(s): Rusconi Filippo | Durand-Dubief Mickaël | Bastin Philippe
Volume: 5
Issue: 1
Year: 2005
Selection and characterization of a promoter for expression of single-copy recombinant genes in Gram-positive bacteria

Author(s): Provvedi Roberta | Maggi Tiziana | Oggioni Marco | Manganelli Riccardo | Pozzi Gianni
Volume: 5
Issue: 1
Year: 2005
Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell

Author(s): Hartshorn Cristina | Anshelevich Aleksandra | Wangh Lawrence
Volume: 5
Issue: 1
Year: 2005
Short description of an alternative simplified method for screening recombinant clones within the "AdEasy-System" by Duplex-PCR

Author(s): Antolovic Dalibor | Koch Moritz | Bohlmann Inga | Kienle Peter | Büchler Markus | Weitz Jürgen
Volume: 5
Issue: 1
Year: 2005
Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

Author(s): Dyson Michael | Shadbolt S Paul | Vincent Karen | Perera Rajika | McCafferty John
Volume: 4
Issue: 1
Year: 2004
Dynamic in vivo imaging and cell tracking using a histone fluorescent protein fusion in mice

Author(s): Hadjantonakis Anna-Katerina | Papaioannou Virginia
Volume: 4
Issue: 1
Year: 2004
Single-track sequencing for genotyping of multiple SNPs in the N-acetyltransferase 1 (NAT1) gene

Author(s): Soucek Pavel | Skjelbred Camilla | Svendsen Marit | Kristensen Tom | Kure Elin | Kristensen Vessela
Volume: 4
Issue: 1
Year: 2004
Identification of proteins in laser-microdissected small cell numbers by SELDI-TOF and Tandem MS

Author(s): Kwapiszewska Grazyna | Meyer Markus | Bogumil Ralf | Bohle Rainer | Seeger Werner | Weissmann Norbert | Fink Ludger
Volume: 4
Issue: 1
Year: 2004
Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells

Author(s): Bento Fernanda | Takeshita Daniela | Sacramento Chester | Machado Tamara | Mathor Monica | Carmona Adriana | Han Sang
Volume: 4
Issue: 1
Year: 2004
Mucosal delivery of anti-inflammatory IL-1Ra by sporulating recombinant bacteria

Author(s): Porzio Stefano | Bossù Paola | Ruggiero Paolo | Boraschi Diana | Tagliabue Aldo
Volume: 4
Issue: 1
Year: 2004
Enhanced cell-permeant Cre protein for site-specific recombination in cultured cells

Author(s): Lin Qing | Jo Daewoong | Gebre-Amlak Kassatihun D | Ruley H Earl
Volume: 4
Issue: 1
Year: 2004
Detecting imbalanced expression of SNP alleles by minisequencing on microarrays

Author(s): Liljedahl Ulrika | Fredriksson Mona | Dahlgren Andreas | Syvänen Ann-Christine
Volume: 4
Issue: 1
Year: 2004
Reduction of arsenic content in a complex galena concentrate by Acidithiobacillus ferrooxidans

Author(s): Makita Mario | Esperón Margarita | Pereyra Benito | López Alejandro | Orrantia Erasmo
Volume: 4
Issue: 1
Year: 2004
Use of polyethyleneimine polymer in cell culture as attachment factor and lipofection enhancer

Author(s): Vancha Ajith | Govindaraju Suman | Parsa Kishore | Jasti Madhuri | González-García Maribel | Ballestero Rafael
Volume: 4
Issue: 1
Year: 2004
A high-throughput cell migration assay using scratch wound healing, a comparison of image-based readout methods

Author(s): Yarrow Justin | Perlman Zachary | Westwood Nicholas | Mitchison Timothy
Volume: 4
Issue: 1
Year: 2004
Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors

Author(s): Douin Victorine | Bornes Stephanie | Creancier Laurent | Rochaix Philippe | Favre Gilles | Prats Anne-Catherine | Couderc Bettina
Volume: 4
Issue: 1
Year: 2004
Investigating the utility of combining Φ29 whole genome amplification and highly multiplexed single nucleotide polymorphism BeadArray™ genotyping

Author(s): Pask Rebecca | Rance Helen | Barratt Bryan | Nutland Sarah | Smyth Deborah | Sebastian Meera | Twells Rebecca | Smith Anne | Lam Alex | Smink Luc | Walker Neil | Todd John
Volume: 4
Issue: 1
Year: 2004
Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR

Author(s): Bubner Ben | Gase Klaus | Baldwin Ian
Volume: 4
Issue: 1
Year: 2004
Quantitative promoter analysis in Physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude

Author(s): Horstmann Verena | Huether Claudia | Jost Wolfgang | Reski Ralf | Decker Eva
Volume: 4
Issue: 1
Year: 2004
Optimization of ectopic gene expression in skeletal muscle through DNA transfer by electroporation

Author(s): Taylor Jordan | Babbs Charlie | Alzghoul Mohammed | Olsen Aaron | Latour Mickey | Pond Amber | Hannon Kevin
Volume: 4
Issue: 1
Year: 2004
A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system

Author(s): Sudomoina Marina | Latypova Ekaterina | Favorova Olga | Golemis Erica | Serebriiskii Ilya
Volume: 4
Issue: 1
Year: 2004
Rapid isolation of yeast genomic DNA: Bust n' Grab

Author(s): Harju Susanna | Fedosyuk Halyna | Peterson Kenneth
Volume: 4
Issue: 1
Year: 2004
Site-specific genomic (SSG) and random domain-localized (RDL) mutagenesis in yeast

Author(s): Gray Misa | Kupiec Martin | Honigberg Saul
Volume: 4
Issue: 1
Year: 2004
A large semi-synthetic single-chain Fv phage display library based on chicken immunoglobulin genes

Author(s): van Wyngaardt Wouter | Malatji Teresiah | Mashau Cordelia | Fehrsen Jeanni | Jordaan Frances | Miltiadou Dubravka | du Plessis Dion
Volume: 4
Issue: 1
Year: 2004
Cell-based assay for the detection of chemically induced cellular stress by immortalized untransformed transgenic hepatocytes

Author(s): Sacco Maria | Amicone Laura | Catò Enrica | Filippini Daniela | Vezzoni Paolo | Tripodi Marco
Volume: 4
Issue: 1
Year: 2004
The defH9-iaaM auxin-synthesizing gene increases plant fecundity and fruit production in strawberry and raspberry

Author(s): Mezzetti Bruno | Landi Lucia | Pandolfini Tiziana | Spena Angelo
Volume: 4
Issue: 1
Year: 2004
A new approach to 'megaprimer' polymerase chain reaction mutagenesis without an intermediate gel purification step

Author(s): Tyagi Rajiv | Lai Richard | Duggleby Ronald
Volume: 4
Issue: 1
Year: 2004
Evaluation of five different cDNA labeling methods for microarrays using spike controls

Author(s): Badiee Azadeh | Eiken Hans | Steen Vidar | Løvlie Roger
Volume: 3
Issue: 1
Year: 2003
Recombining overlapping BACs into a single larger BAC

Author(s): Kotzamanis George | Huxley Clare
Volume: 4
Issue: 1
Year: 2004
Expressing functional siRNAs in mammalian cells using convergent transcription

Author(s): Tran Nham | Cairns Murray | Dawes Ian | Arndt Greg
Volume: 3
Issue: 1
Year: 2003
Performance of high-throughput DNA quantification methods

Author(s): Haque Kashif | Pfeiffer Ruth | Beerman Michael | Struewing Jeff | Chanock Stephen | Bergen Andrew
Volume: 3
Issue: 1
Year: 2003
Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Author(s): Ponchel Frederique | Toomes Carmel | Bransfield Kieran | Leong Fong | Douglas Susan | Field Sarah | Bell Sandra | Combaret Valerie | Puisieux Alain | Mighell Alan | Robinson Philip | Inglehearn Chris | Isaacs John | Markham Alex
Volume: 3
Issue: 1
Year: 2003
Quantitative Real Time Polymerase Chain Reaction for measurement of human Interleukin – 5 receptor alpha spliced isoforms mRNA

Author(s): Pérez Claudina | Vandesompele Jo | Vandenbroucke Ina | Holtappels Gabriele | Speleman Frank | Gevaert Philippe | Van cauwenberge Paul | Bachert Claus
Volume: 3
Issue: 1
Year: 2003
In vivo mucosal delivery of bioactive human interleukin 1 receptor antagonist produced by Streptococcus gordonii

Author(s): Ricci Susanna | Macchia Giovanni | Ruggiero Paolo | Maggi Tiziana | Bossù Paola | Xu Li | Medaglini Donata | Tagliabue Aldo | Hammarström Lennart | Pozzi Gianni | Boraschi Diana
Volume: 3
Issue: 1
Year: 2003
Coupling of importin beta binding peptide on plasmid DNA: transfection efficiency is increased by modification of lipoplex's physico-chemical properties

Author(s): Carrière Marie | Escriou Virginie | Savarin Aline | Scherman Daniel
Volume: 3
Issue: 1
Year: 2003
Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse

Author(s): Wang Weigang | Wyckoff Jeffrey | Wang Yarong | Bottinger Erwin | Segall Jeffrey | Condeelis John
Volume: 3
Issue: 1
Year: 2003
Hydrodynamic guiding for addressing subsets of immobilized cells and molecules in microfluidic systems

Author(s): Brevig Thomas | Krühne Ulrich | Kahn Rachel | Ahl Thomas | Beyer Michael | Pedersen Lars
Volume: 3
Issue: 1
Year: 2003
An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography

Author(s): Scheich Christoph | Sievert Volker | Büssow Konrad
Volume: 3
Issue: 1
Year: 2003
A fluorescent cassette-based strategy for engineering multiple domain fusion proteins

Author(s): Truong Kevin | Khorchid Ahmad | Ikura Mitsuhiko
Volume: 3
Issue: 1
Year: 2003
The Flp double cross system a simple efficient procedure for cloning DNA fragments

Author(s): Sadowski Paul
Volume: 3
Issue: 1
Year: 2003
Expression of self-complementary hairpin RNA under the control of the rolC promoter confers systemic disease resistance to plum pox virus without preventing local infection

Author(s): Pandolfini Tiziana | Molesini Barbara | Avesani Linda | Spena Angelo | Polverari Annalisa
Volume: 3
Issue: 1
Year: 2003
Oligodeoxyribonucleotide probe accessibility on a three-dimensional DNA microarray surface and the effect of hybridization time on the accuracy of expression ratios

Author(s): Dorris David | Nguyen Allen | Gieser Linn | Lockner Randall | Lublinsky Anna | Patterson Marcus | Touma Edward | Sendera Timothy | Elghanian Robert | Mazumder Abhijit
Volume: 3
Issue: 1
Year: 2003
In situ transduction of target cells on solid surfaces by immobilized viral vectors

Author(s): Hobson David | Pandori Mark | Sano Takeshi
Volume: 3
Issue: 1
Year: 2003
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