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α-Tocopherol levels in natural and artificial aging of soybean seeds=Teores de α-tocoferol no envelhecimento natural e artificial de sementes de soja

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Author(s): Maria Izabel Krüger Giurizatto | Osvaldo Ferrarese-Filho | Maria de Lourdes Lucio Ferrarese | Antonio Dias Robaina | Manoel Carlos Gonçalves | Cláudia Andréa Lima Cardoso

Journal: Acta Scientiarum : Agronomy
ISSN 1679-9275

Volume: 34;
Issue: 3;
Start page: 339;
Date: 2012;
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Keywords: (Glycine max (L.) Merrill) | α-tocopherol | storage | (Glycine max (L.) Merrill) | α-tocoferol | armazenamento.

ABSTRACT
Tocopherols are well known constituents of vitamin E, and the main antioxidants in soybean. Are natural antioxidants and stabilizers that can inhibit lipid degradation, reducing non-enzymatic oxidation of these compounds during storage of seeds, germination and initial development of seedlings. The objective of this work was to determine the level of α-tocopherol in four soybean seeds cultivars naturally and artificially aged. Seeds of four soybean cultivars stored from 0 to 180 days in a dry chamber (natural aging) and subjected to high temperature and humidity (artificial aging) were analyzed for α-tocopherol content. The quantification of -tocopherol in the soybean seeds was performed by high performance liquid chromatography (HPLC) combined with the Soxhlet extraction method. Significant differences in α-tocopherol levels in seeds were observed for all cultivars and storage times. The α-tocopherol contents of the soybean seeds showed linear correlations with an increasing period of storage for all the cultivars studied. However, the artificially aged seeds had a higher content of α-tocopherol than those naturally aged. Tocoferóis são conhecidos constituintes da vitamina E, sendo os principais antioxidantes lipofílicos na soja. Eles são substâncias antioxidantes naturais e estabilizadoras, capazes de inibir a degradação de lipídios limitando a oxidação não enzimática destes compostos durante o armazenamento das sementes, a germinação e o desenvolvimento inicial das plântulas. O objetivo deste trabalho foi determinar o conteúdo de α-tocoferol nas sementes de quarto cultivares de soja envelhecidas natural e artificialmente. As sementes de quatro cultivares de soja envelhecidas em armazenamento de zero a 180 dias em câmara seca (envelhecimento natural) e submetidas a altas temperaturas e umidade relativa (envelhecimento artificial) foram analisadas quanto ao teor de α-tocoferol. A quantificação de -tocoferol nas sementes de soja foi efetuada por cromatografia líquida de alta eficiência (CLAE) associada ao método de extração Soxhlet. Diferenças significativas nos teores de -tocoferol nas sementes foram observadas para todas as cultivares e tempos de armazenamento. O teor de α-tocoferol das sementes de soja mostrou resposta linear crescente com o aumento dos períodos de armazenamento. Entretanto, as sementes envelhecidas artificialmente apresentaram maior teor de α-tocoferol do que as envelhecidas naturalmente.
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