Academic Journals Database
Disseminating quality controlled scientific knowledge

The abundance of short proteins in the mammalian proteome.

Author(s): Frith Martin C | Forrest Alistair R | Nourbakhsh Ehsan | Pang Ken C | Kai Chikatoshi | Kawai Jun | Carninci Piero | Hayashizaki Yoshihide | Bailey Timothy L | Grimmond Sean M

Journal: PLoS Genetics
ISSN 1553-7390

Volume: 2;
Issue: 4;
Start page: e52;
Date: 2006;
Original page

Short proteins play key roles in cell signalling and other processes, but their abundance in the mammalian proteome is unknown. Current catalogues of mammalian proteins exhibit an artefactual discontinuity at a length of 100 aa, so that protein abundance peaks just above this length and falls off sharply below it. To clarify the abundance of short proteins, we identify proteins in the FANTOM collection of mouse cDNAs by analysing synonymous and non-synonymous substitutions with the computer program CRITICA. This analysis confirms that there is no real discontinuity at length 100. Roughly 10% of mouse proteins are shorter than 100 aa, although the majority of these are variants of proteins longer than 100 aa. We identify many novel short proteins, including a "dark matter" subset containing ones that lack detectable homology to other known proteins. Translation assays confirm that some of these novel proteins can be translated and localised to the secretory pathway.

Tango Rapperswil
Tango Rapperswil

RPA Switzerland

RPA Switzerland

Robotic process automation