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Actividad bacteriostática y bactericida de la tintura de propóleos sobre bacterias enteropatógenas

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Author(s): Marielsa Gil | Amarily Perelli | Riloarbert, Alvarado | Yuneidi Arias | Eucaris Blumentha

Journal: Salus Online
ISSN 1316-7138

Volume: 16;
Issue: 3;
Start page: 29;
Date: 2012;
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Keywords: Propolis tincture | minimum inhibitory concentration | minimum bactericidal concentration | enteropathogenic bacteria

ABSTRACT
Propolis is a resinous substance produced by adult bees Apis mellífera to ensure complete asepsis of the hive. Other species of bees also produce propolis. The aim of this study was to assess the bacteriostatic and bactericidal activity in vitro of commercial ethanolic tincture of propolis 70% v/v from an apiary in Cojedes State, Venezuela on enteropathogenic bacteria ATCC. Used Salmonella enterica subsp enterica suptype paratyphi A, Salmonella enterica subsp enterica suptype paratyphi B, Salmonella enterica subsp enterica subtype typhi, Shigella sonnei, Shigella flexneri, Yersínia enterocolitica, and Escherichia coli (O157:H7). The strains mentioned were exposed to different concentrations of propolis for 24 hours to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the macrodilution method in tubes. Results: S. paratyphi A was the only bacterium with a total inhibitory effect on agar BHI. In other bacteria, a partial bacteriostatic effect was observed. Y. enterocolítica was the most sensitive with a CMI 4% and CMB 8 %, followed by E. coli (O157:H7). S. typhi, S. paratyphi B and S. sonnei with CMI 8% and CMB 11% and finally S. flexneri and S. paratyphi A were the most resistant with CMI 11% and CMB 15%. Results indicate that propolis tincture has a bacteriostatic and bactericidal effect in vitro in the strains studied, and that such effect is not due to the ethanol present as solvent.
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