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Adiponectin Effect on The Viability of Human Endometrial Stromal Cells and mRNA Expression of Adiponectin Receptors

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Author(s): Somayeh Bohlouli | Mozafar Khazaei | Masoud Teshfam | Hosein Hassanpour

Journal: International Journal of Fertility & Sterility
ISSN 2008-076X

Volume: 7;
Issue: 1;
Start page: 43;
Date: 2013;
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Keywords: Adiponectin | Stromal Cells | Adipo R1 | Adipo R2 | Endometrium

ABSTRACT
Background: Adiponectin is one of the most important adipokines secreted from fattytissue that has a direct inhibitory effect on the development of cancer cells. Adiponectinplays an important role in human reproduction system and fertility of women. Adiponectinconcentration decreases in women with endometriosis and endometrial cancer.The aim of the present study was to investigate the effect of adiponectin on humanendometrial stromal cell (HESC) viability as well as mRNA expression of Adipo R1and Adipo R2 receptors.Materials and Methods: In this experimental study, eight endometrial biopsies weretaken and stromal cells were separated by enzymatic digestion and cell filtrations. Stromalcells of each biopsy were divided into four groups: control, 10, 100, and 200 ng/mladiponectin concentrations. The effect of adiponectin on viability of the normal HESCswas studied by trypan blue staining and the relative expression levels of Adipo R1 andR2 were analyzed by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR). Data were analyzed by one way ANOVA and unpaired student’s t test andp
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