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Ameliorative Effect of Two Chosen Seaweeds Kappaphcus alverizii and Cymodocea serrulata against Vibrio Pathogens

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Author(s): Rathna Kumari. P | Balasundaram. A | Masilamani.V | Kolanchinathan. P | George John

Journal: Advanced Biotech
ISSN 0973-0109

Volume: 12;
Issue: 07S;
Start page: 30;
Date: 2013;
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Keywords: Kappaphcus alverizii | Cymodocea serrulata | Disc diffudion | Well diffudion Vibrio alginolyticus | Vibrio vulnificus | Vibrio parahaemolyticus | Vibrio fluvialis.

ABSTRACT
In the present study we have evaluated the antibacterial activity of Kappaphcus alverizii and Cymodocea serrulata against four pathogenic bacteria Vibrio alginolyticus, Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio fluvialis which cause vibriosis in the shrimps. Two seaweeds Kappaphcus alverizii and Cymodocea serrulata were collected from the coast of Devipattinam in Pudukottai district. Seaweeds were identified in CMFRI, Mandapam and the specimens were stored for further use. Collected seaweeds were dried in shade and ground into fine powder and stored in a closed container for further use. Extract was prepared using three different solvents, butanol, hexane and ethanol in soxhlet apparatus. The axenic cultures were obtained from the Institute of Microbial Type Culture collection (MTCC) Chandigarh. It was subcultured and used for the antibacterial assay. Four pathogenic bacteria Vibrio alginolyticus, Vibrio vulnificus, Vibrio parahaemolyticus and Vibrio fluvialis which cause vibriosis in the shrimps were obtained. Laboratory investigations were carried out to infer the antimicrobial activity of two seaweeds against prawn pathogens by well diffusion and disc diffusion methods. Among the three solvents hexane extract produced maximum inhibitory zone in disc diffusion methods against V. parahaemolyticus, in well diffusion method hexane and butanolic extract inhibited V. fluvialis with 25mm inhibitory zone. Phytochemical studies were also carried out. The chemical components found in the butanolic extract of Kappaphcus alverizii and Cymodocea serrulata. There were found to be flavanoids, tannins, and carbohydrates in Kappaphcus alverizii. It was found that reducing sugar, carbohydrates, alkaloids, saponins and flavonoids were present in extract of Cymodocea serrulata.

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