Academic Journals Database
Disseminating quality controlled scientific knowledge

Amplification, cloning and expression of Brucella melitensis bp26 gene (OMP28) isolated from Markazi province (Iran) and purification of Bp26 Protein

ADD TO MY LIST
 
Author(s): Hosseini, S.D. | Azizpour, M. | Akbari, N. | Basiri, H. | Behrozikhah, A.M. | Eskandari, S.

Journal: Archives of Razi Institute
ISSN 0365-3439

Volume: 68;
Issue: 2;
Start page: 111;
Date: 2013;
Original page

Keywords: Brucella melitensis | bp26 gene | OMP28 | brucellosis | cloning | experssion

ABSTRACT
Brucellosis is a debilitative disease that imposes costs on both economy and society. It is shown that although the vaccine can prevent abortion, it does not provide complete protection against infection. In Iran, Brucella melitensis is a common causative agent for brucellosis and BP26 protein of this bacterium having a good antigenesity and an important vaccine candidate. In this study B. melitensis bp26 gene was cloned first in to PTZ57R/T vector and accessed on the PET28a vector and sequenced. Recombinant vector transformed and expressed in to E. coli BL21 (DE3) and then recombinant protein was purified with Ni-NTA column of chromatography against His tag. Obtained rOmp28 could be used as a research experimental tool to find its potential as a detection kit and vaccine candidate.
RPA Switzerland

Robotic Process Automation Switzerland

    

Tango Rapperswil
Tango Rapperswil