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Analysis of occult hepatitis B virus infection among blood donors in Tangshan, China

Author(s): CAO Xiao

Journal: Journal of Clinical Hepatology
ISSN 1001-5256

Volume: 29;
Issue: 2;
Start page: 117;
Date: 2013;
Original page

Keywords: hepatitis B | hepatitis B virus | enzyme-linked immunosorbent assay

ObjectiveTo investigate the prevalence of occult hepatitis B virus (HBV) infection (OBI) among the volunteer blood donors in Tangshan, China. MethodsEnzyme-linked immunosorbent assay was used to detect serum HBV markers in the blood donors. Nucleic acid test (NAT) was performed in the hepatitis B surface antigen (HBsAg)-negative blood samples to detect HBV DNA, and Roche reagent was used to measure the viral load of HBV DNA in NAT-positive samples. ResultsAmong the HBsAg-negative blood samples of 116 741 blood donors, 39 (0.033%) had positive results in NAT, and 35 (0.029%) were confirmed with OBI. The viral load of HBV DNA was less than 102 IU/ml in 97.1% of the blood donors with OBI. Of the blood donors with OBI, 27 (77.1%) were positive for at least one of hepatitis B surface antibody (HBsAb), hepatitis B e antigen, hepatitis B e antibody, and hepatitis B core antibody (HBcAb), and 8 (22.9%) were negative for all serum HBV markers. Of the 27 positive blood donors, 22 (81.5%) were positive for HBcAb, and 15 (55.6%) were positive for HBsAb. ConclusionOBI occurs in some HBsAg-negative blood donors in Tangshan, and the viral load of HBV DNA is low. NAT is effective in increasing the detection rate of HBV infection.
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