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Analyzing the differences in the relative lengths of DNA telomeres between the aging and the neonatal Chinese alligators

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Author(s): XU Min | WU Xiao-Bing

Journal: Acta Zoologica Sinica
ISSN 0001-7302

Volume: 54;
Issue: 6;
Start page: 1119;
Date: 2008;
Original page

Keywords: Chinese alligator | Telomere | Lifespan | Polymerase chain reaction

ABSTRACT
To assess the differences in the relative lengths of the DNA telomere between the matured and the adolescent Chinese alligators(Alligator sinensis)and to offer the possible molecular evidence for judging the age of the Chinese alligators,we applied the PCR method to amplify the telomere DNA of both the matured and the juvenile Chinese alligators In our research. Subsequently, we analyzed and compared the outcomes of the two groups by using a Semi-quantitative technique. The results of our analyses show that the relative average gray value of the blood cells' telomere PCR products of the adolescent group is higher than that of the matured group. These analytical results are indications that the blood cells' telomere DNA sequence of the adolescent Chinese alligators is longer than that of the matured Chinese alligators. Our paper will also discuss the feasibility of the use of the PCR method to determine the relative lengths of the telomere [Acta Zoologica Sinica 54(6):1119–1123, 2008]. 
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