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Antagonistic effects of vitamin E on transcription activity cyP450 and antioxidant enzyme genes in the liver of mice treated acutely with TCDD

Author(s): YIN Hai-Ping | XU Jian-Ping | YANG Shou-Hua | ZHOU Xian-Qing

Journal: Acta Zoologica Sinica
ISSN 0001-7302

Volume: 54;
Issue: 6;
Start page: 1038;
Date: 2008;
Original page

Keywords: TCDD | Vitamin E | CYP450 gene | Mice | Peroxydase

To investigate the effects of vitamin E on transcription activity of liver CYP4501A1,CYP4501A2,CYP4501B1,SOD,GSH-Px,CAT gene in mice treated acutely with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD). The acute treatment was assigned 3 groups, and the level of both TCDD and vitamin E were 0 and 0, 30 and 0, 30 μg/kg and 100 mg/kg·d, respectively. Livers were collected to analyze transcription activity of CYP4501A1, CYP4501A2, CYP4501B1, SOD, GSH-Px, CAT gene by Real-time PCR method. The results showed that 1)the level of CPY4501A1, 1A2, 1B1 in the acute TCDD-treatment were significant higher than the that of control group , vitamin E(100 mg/kg) inhibited the significant increase; 2)there was a decreasing tendency of SOD, GSH-Px mRNA levels and significant decrease of CAT mRNA level after acute treatment with TCDD, the mRNA level of SOD and CAT had significant increase, while the level of GSH-Px had a increasing tendency in the group supplemented vitamin E 100 mg/kg compared to the group treated with TCDD. These results suggested that TCDD can affect or partly affect transcription activity of liver CYP450 genes and antioxidant enzyme gene, in mice, while vitamin E can alleviate or partly alleviate the TCDD effects[Acta Zoologica Sinica 54(6): 1038–1043, 2008].
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