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泥螺多肽抗肿瘤活性的初步研究 Anti-Tumor Activity of the Peptide from Bullacta exarata

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Author(s): 林焕乐 | 励芝伊 | 陈博 | 易忠达 | 王泽峰 | 马剑茵

Journal: Hans Journal of Biomedicine
ISSN 2161-8976

Volume: 02;
Issue: 03;
Start page: 19;
Date: 2012;
Original page

Keywords: 泥螺多肽 | 抗肿瘤 | 酶解 | 前列腺癌 | Peptides of Bullacta Exarata | Anti-Tumor | Enzymatic | Prostate Cancer

ABSTRACT
目的:提取分离泥螺软体组织,探索其体外抗肿瘤活性。方法采用胰蛋白酶酶解法获得泥螺多肽粗提物,通过超滤膜滤过技术获得分子量在5~10 kDa的组分。用葡聚糖凝胶G-75分离、纯化,MTT法测定其体外抗肿瘤活性并用荧光染色法观察细胞形态。结果经膜分离得到的组分(分子量5~10 KD)和经葡聚糖凝胶G-75分离得到的泥螺多肽对前列腺PC-3肿瘤细胞均有一定的抑制作用,且后者强于前者。结论泥螺多肽具有一定的抗前列腺PC-3肿瘤细胞的活性。   Objective: To isolate and purify the peptide from Bullacta exarata, and study its anti-tumor effect on prostate cancer. Methods: The antimicrobial peptides were hydrolyzed from soft tissues of Bullacta exarata by trypsin, and purified by sephadex chromatography. Measured by MTT assay in vitro anti-tumor activity and cell morphology was observed by fluorescence staining. Results: The components separated from membrane (molecular weight 5 - 10 KD)and isolated by the Sephadex G-75 could inhibitse the PC-3 cells growth, and the latter stronger than the former. Conclusion: The Bullacta peptides could inhibitse the PC-3 cells growth and have anti-tumor activities on prostate cancer.

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