Academic Journals Database
Disseminating quality controlled scientific knowledge

Antibacterial activity of essential oil of Baccharis dracunculifolia DC (Asteraceae) and its active compound nerolidol in combination to EDTA or lysozyme

ADD TO MY LIST
 
Author(s): Jane Mary Lafayette Neves Gelinski | Juliana Carla Dalla Rosa | Elisangela de Fátima Aparecida Paravisi Duquesne | Cesar Milton Baratto

Journal: Evidência : Ciência e Biotecnologia - Interdisciplinar
ISSN 1519-5287

Volume: 7;
Issue: 2;
Start page: 131;
Date: 2007;
Original page

Keywords: Antibacterial | Essential oil | Diffusion in Agar | Nerolidol.

ABSTRACT
Essential oils of plants are considered sources of biological active substances. The family Asteraceae has been widely studied in relation to its chemical composition and biological activity. In the genus Baccharis the flavonoids and terpenoids are the most commonly studied compounds. B. dracunculifolia DC, is native to the Brazilian cerrado and considered the main botanical source of green propolis. The aims of this study were to evaluate the antibacterial activity of the essential oil from leaves and flowers of B. dracunculifolia and its active compound, the nerolidol. This compound was utilized pure or in combined form with EDTA or with lysozyme against Gram positive and negative pathogenic bacteria strains. The techniques of well diffusion agar and disc diffusion filter paper were utilized to test antibacterial activity of the following substances: essential oil of B. dracunculifolia (Ole30), pure nerolidol or combined with EDTA or lysozyme, and different antibiotics used as control: gentamicin, tetracycline, streptomycin, oxacillin, chloramphenicol, vancomicin, penicillin, amoxicillin, rifampin and erythromycin. The most inhibited bacteria by the Ole30 were: Proteus sp., Staphylococcus sp, Escherichia coli and Listeria monocytogenes. In well diffusion assay, both Ole30 and chloramphenicol inhibited Proteus sp., Staphylococcus sp. and L. monocytogenes but Salmonella enterica subsp. enterica Typhimurium and S. Panama were resistant to the Ole30. In both techniques Staphylococcus aureus ATCC 25923 was resistant to the Ole30. The nerolidol inhibited Staphylococcus sp. and L. monocytogenes, and combined with EDTA inhibited Proteus sp. and combined with lysozyme inhibited S. aureus ATCC 25923 and L. monocytogenes.
RPA Switzerland

RPA Switzerland

Robotic process automation

    

Tango Jona
Tangokurs Rapperswil-Jona