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Antibacterial potential of Azadirachta indica seed and Bacopa monniera leaf extracts against multidrug resistant Salmonella enterica serovar Typhi isolates

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Author(s): Shyamapada Mandal | Manisha Deb Mandal | Nishith Kumar Pal

Journal: Archives of Medical Science
ISSN 1734-1922

Volume: 3;
Issue: 1;
Start page: 14;
Date: 2007;
Original page

Keywords: bactericidal | plant extracts | time kill | typhoidal bacteria

ABSTRACT
Introduction: Neem (Azadirachta indica) and brahmi (Bacopa monniera) are well known medicinal plants, but their antibacterial activity against the typhoidal pathogen Salmonella enterica serovar Typhi has not been studied. Material and methods: A. indica seed and B. monniera leaf extracts were assayed for antibacterial activity by agar well diffusion and agar dilution methods in order to determine the zone diameter of inhibition and minimum inhibitory concentration (MIC) values, respectively. Killing efficacy of the extracts was determined at various concentrations in Mueller-Hinton broth. Time-dependent killing was achieved using 500 µg/ml (1× MIC) of the extract for S. enterica serovar Typhi strain. Results: The A. indica seed and B. monniera leaf extracts showed excellent antibacterial activity against the isolates having zone diameter of inhibition 9-19 mm and 9-18 mm. The MICs for the isolates were in the range 50-500 µg/ml (A. indica extract) and 50-600 µg/ml (B. monniera extract). The A. indica and B. monniera extracts were bactericidal against S. enterica serovar Typhi at concentrations of 400 µg/ml and 450 µg/ml, respectively. A. indica and B. monniera against the test microorganisms displayed significant antibacterial (at concentrations ≥250 and ł300 µg/ml, respectively) as well as time killing (at 500 µg/ml) activities (p
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