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Antioxidant and Anti-Tyrosinase Activity from Olea Leaf Extract Depended on Seasonal Variations and Chromagraphy Treatment

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Author(s): Dao-Mao Yang | Ming-An Ouyang

Journal: International Journal of Organic Chemistry
ISSN 2161-4687

Volume: 02;
Issue: 04;
Start page: 391;
Date: 2012;
Original page

Keywords: Olive Leaf Extract | Antioxidant Activity | Tyrosinase Inhibitory Activity | Non-Competitive Competition | Chromagraphy Treatment

ABSTRACT
In the present study, olive leaf extract, harvested from four seasons, extracted with solvent (methanol and water), was treated by D101 and Sephadex LH-20, and divided into 10 fractions by TLC detection. The antioxidant activity was evaluated using DPPH, superoxide anion scavenging and reducing power assays. Tyrosinase inhibitory activity was also performed with L-DOPA as substrate. On extraction method, there is no significant difference in methanol and water. Among 12 months, autumn showed the best harvest time in whole. OLE showed 90% tyrosinase inhibitory activity and inhibition type was non-competitive competition. By chromagraphy treatment, the ingredients of polysacchmaride and protein showed high bioactivity towards antioxidant and anti-tyrosinase activity, the rest part showed different extent of activity and prove some effect by chromatography treatment indirectly. OLE might be a valuable bioactive source and can be applicable in both the cosmetic and medical use.
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