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Application to Dissolution Assessments; A Facile and Rapid procedure of Atenolol and Hydrochlorothiazide in Pharmaceutical Formulation by RP-HPLC assay Method

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Author(s): M.C. Sharma, S. Sharma*, A.D. Sharma

Journal: Drug Invention Today
ISSN 0975-7619

Volume: 3;
Issue: 6;
Start page: 100;
Date: 2011;
Original page

Keywords: Atenolol | Hydrochlorothiazide | RP-HPLC | Analytical method validation

ABSTRACT
A RP-HPLC method was developed and validated for quantitative determination of Atenolol and Hydrochlorothiazide in pharmaceutical dosage forms. The chromatography was carried out on a 25 cm × 4.6 mm i.d, 5-μm particle, Phenomenex Luna C18 column under reversed-phase partition chromatographic conditions. The mobile phase was a mixture of ammonium acetate (0.02M, pH 4.0 adjusted with glacial acetic acid) and acetonitrile in ratio of 40:60 (v/v) at a flow rate of 1.0ml/min. Clarithromycin was used as an internal standard. The linearity was established in the range of 20 - 400 ng/ml Atenolol and 5-150 ng/ml Hydrochlorothiazide for HPLC. This validated method can be used for estimation of Atenolol and Hydrochlorothiazide in pharmaceutical suspension.
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