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Application of Response Surface Methodology for the Determination of Optimum Reaction Conditions (Temperature and pH) for Starch Hydrolysis by a-Amylase

Author(s): Nivedita Jaiswal | Om Prakash | Mahe Talat | S.H. Hasan | Rajesh Kumar Pandey

Journal: Asian Journal of Biochemistry
ISSN 1815-9923

Volume: 6;
Issue: 4;
Start page: 357;
Date: 2011;
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Keywords: optimum reaction conditions | hydrolysis | starch | temperature | pH

2 = 97.85%) with the experimental results indicating the applicability of proposed model. The multiple regression Analysis and ANOVA showed the individual and cumulative effect of pH and temperature on enzyme activity indicating that the activity increased with the increase of pH unto 5.5 and temperature 75C. Thus, the RSM was successful in determining the optimum reaction conditions for starch hydrolysis by a-amylase.]]>
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