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Biodegradation of Organophosphate Pesticide by Soil Fungi

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Author(s): Harish.R | Supreeth.M | Jyoti Bala Chauhan

Journal: Advanced Biotech
ISSN 0973-0109

Volume: 12;
Issue: 09;
Start page: 04;
Date: 2013;
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Keywords: Organophosphorous pesticide | Bioremediation | Gas chromatography | Rhizopus nodosus | Trichoderma harznaium

ABSTRACT
In this study using enrichment technique biodegradation of organophosphorous pesticide, (Chloropyrifos and Ethion) was performed. Two fungal isolates Trichoderma harznaium and Rhizopus nodosus isolated from the contaminated soil by enrichment technique, was capable of metabolizing the organophosphorous insecticide (Chloropyriofos and Ethion) as a sole carbon source when grown in burkes mineral medium at concentration of 100 PPM respectively. Fungi were able to degrade 70-80% of the parental molecule (technical grade) in 21 days period of incubation. Further, efficiency of degradation was increased by 10-20% with the supplementation of 0.1% dextrose to the mineral media. Desertion of Chloropyrifos, Ethion and its metabolites were analyzed by thin layer chromatography and gas Chromatography. It was evident from the result that the isolated fungi could be used to bioremediate the area contaminated with organophosphorous pesticide.
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