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Biological Behavior of MG63 Cells on the Hydroxyapatite Surface

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Author(s): Young Hwa Ko | Dong Seok Seo | Jong Kook Lee

Journal: Bioceramics Development and Applications
ISSN 2090-5017

Volume: 1;
Start page: 4 pages;
Date: 2011;
Original page

Keywords: hydroxyapatite | tuna bone | bone ash | cell proliferation

ABSTRACT
The response of human bone marrow cell to bone ash-derived hydroxyapatite (HA) and tuna bone-derived HA powders was compared. HA ceramics were prepared from the commercial bone ash and waste of tuna bone. HA powders were prepared by soaking the bone ash and tuna bone in 0.1M of NaOH solution at 80°C for 4 hours. Both powders were calcined at 800°C for 1 hour to completely remove organic and were attritor-milled for 24 hours. The bone ash-derived HA (AHA) and tuna bone-derived HA (THA) ceramics were prepared by cold isostatically pressed and sintered 1200°C with a dwell time of 1 hour. A human bone cell line MG-63 cells were used to test biocompatibility of AHA and THA ceramics. Cell suspensions in DMEM containing 10% FBS and 1% penicillin-streptomycin were seeded onto 24-well plate containing THA and AHA ceramics. Cell proliferation was evaluated by MTS assay, and cell morphology was observed by SEM.
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