Author(s): F. M. M. T. Marikar
Journal: MicologĂa Aplicada Internacional
ISSN 1534-2581
Volume: 21;
Issue: 2;
Start page: 63;
Date: 2009;
Original page
ABSTRACT
An alternative culture medium to grow Colletotrichum gloeosporioides on coconut watery endosperm agar and broth was developed. The medium is based upon the watery endosperm from Cocos nucifera, which contains nutrients promoting mycelial growth and sporulation. Sucrose (40%) further enhanced fungal growth in the broth. Dilution of watery endosperm reduced mycelial growth rate by 50%. The coconut watery endosperm is cheaper and more easily available than commercial potato dextrose agar medium in tropical regions.
Journal: MicologĂa Aplicada Internacional
ISSN 1534-2581
Volume: 21;
Issue: 2;
Start page: 63;
Date: 2009;
Original page
ABSTRACT
An alternative culture medium to grow Colletotrichum gloeosporioides on coconut watery endosperm agar and broth was developed. The medium is based upon the watery endosperm from Cocos nucifera, which contains nutrients promoting mycelial growth and sporulation. Sucrose (40%) further enhanced fungal growth in the broth. Dilution of watery endosperm reduced mycelial growth rate by 50%. The coconut watery endosperm is cheaper and more easily available than commercial potato dextrose agar medium in tropical regions.