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A comparative phylogenetic analysis of Theileria spp. by using two "18S ribosomal RNA" and "Theileria annulata merozoite surface antigen" gene sequences

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Author(s): Habibi, G.

Journal: Archives of Razi Institute
ISSN 0365-3439

Volume: 68;
Issue: 1;
Start page: 47;
Date: 2013;
Original page

Keywords: Theileria annulata | Tams1 | 18S rRNA | Taxonomy | Phylogenetic analysis

ABSTRACT
More than 185 species, strains and unclassified Theileria parasites are categorized in the Entrez Taxonomy. The accurate diagnosis and proper identification of the causative agents are important for understanding the epidemiology, prevention and appropriate treatment. This study aims to discuss the importance of twogenes of Theileria annulata 18S ribosomal RNA (18S rRNA) and Theileria annulata merozoite surface antigen (Tams1) for phylogenetic analysis of molecular data. Eight Theileria annulata isolates including S15 Iran vaccine strain, six T. annulata field isolates and one Iraq T. annulata field isolate were studied inthis study. The 18S rRNA and Tams1 gene sequences were investigated by phylogenetic analysis after DNA extraction, PCR, and DNA sequencing. Sequence fragments of about 1410 to 1412 bp of thecomplete 18S rRNA gene and 778 nucleotides of Tams1 gene sequences were used in the analysis of nucleotide diversity. Sequence alignment and phylogenetic analysis were performed by constructing the phylogenetic tree and identity matrix based on two above genes. The results of the study revealed, the 18S rRNA is informative in estimation of relationships among Theileria species and strains, but the Tams1 seems to be appropriate for analysis of the molecular antigenicity in vaccine development.
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