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Comparison of phenotypic and molecular tests to identify lactic acid bacteria

Author(s): Paula Mendonça Moraes | Luana Martins Perin | Abelardo Silva Júnior | Luís Augusto Nero

Journal: Brazilian Journal of Microbiology
ISSN 1517-8382

Volume: 44;
Issue: 1;
Start page: 109;
Date: 2013;
Original page

Keywords: lactic acid bacteria | identification | 16sDNA | PCR | Biolog | API

Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
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