Author(s): Paula Mendonça Moraes | Luana Martins Perin | Abelardo Silva Júnior | Luís Augusto Nero
Journal: Brazilian Journal of Microbiology
ISSN 1517-8382
Volume: 44;
Issue: 1;
Start page: 109;
Date: 2013;
Original page
Keywords: lactic acid bacteria | identification | 16sDNA | PCR | Biolog | API
ABSTRACT
Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
Journal: Brazilian Journal of Microbiology
ISSN 1517-8382
Volume: 44;
Issue: 1;
Start page: 109;
Date: 2013;
Original page
Keywords: lactic acid bacteria | identification | 16sDNA | PCR | Biolog | API
ABSTRACT
Twenty-nine lactic acid bacteria (LAB) isolates were submitted for identification using Biolog, API50CHL, 16S rDNA sequencing, and species-specific PCR reactions. The identification results were compared, and it was concluded that a polyphasic approach is necessary for proper LAB identification, being the molecular analyzes the most reliable.
