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Concentration of nitric oxide in patients’ saliva from various metal restorative activities in the oral cavity

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Author(s): Enes Pašić | Amira Dedić | Jasminko Huskić | Sanja Hadžić

Journal: Acta Medica Academica
ISSN 1840-1848

Volume: 40;
Issue: 2;
Start page: 159;
Date: 2011;
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Keywords: Saliva | Nitric oxide | Various metals

ABSTRACT
Objective. Th e aim of this paper is to determine the concentration ofnitric oxide (NO) in the saliva of subjects resulting from the presence of various metal restorative activities in the oral cavity. Patients and methods. Th e research was conducted on 20 subjects (12 women and 8 men), aged 18-30, with no metal fillings in their oral cavity and 20 subjects (11 women and 9 men) aged 18-30 with prosthetic and conservative work in their oral cavities made of various metals. Th e pH of the saliva was established by means of an “Orion” type pH meter at an optimal temperature of 250C. Th e concentration of NO was determined by conversion of NO3-2 into NO2-2 with the help of elementary zinc and then by colorimetric testing of the NO2-2 concentration by means of the Griess reagent. Results. Our results established that the concentration of NO in patients with various metals (60.18±10.24) is 33.7% higher, which is statistically insignificant, in relation to the control group (45.01±6.28). Conclusion. Various metal restorative activities stemming from dentistry practice do not cause changes in the pH values of saliva and NO concentrations.
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