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Culture Conditions Affect the Category and Production of Ubiquinones in a Recombinant Escherichia Coli with an Exogenous Decaprenyl Diphosphate Synthase

Author(s): Jiazhou Li | Yuanyuan Zhang | Mingquan Xie | Xiaochun Luo

Journal: Advance Journal of Food Science and Technology
ISSN 2042-4868

Volume: 5;
Issue: 9;
Start page: 1186;
Date: 2013;
Original page

Keywords: Decaprenyl diphosphate synthase | Escherichia coli | Rhizobium radiobacter | ubiquinone

Ubiquinones (UQ) are important electron transporters and play lot of important roles in most organisms. In different species, UQ was classified to be UQ-6, 7, 8, 9, 10 according to their polyprenyl side chain length. The side chain’s length is determined by the enzyme named Poly-Prenyl diphosphate Synthases (PPPS). Bacteria are usually reconstructed to producing UQ-10 used in human’s food additive, medicine or cosmetics, such as using decaprenyl Diphosphate Synthase (DPS) gene from R. radiobacter to substitute E. coli’s octaprenyl diphosphate synthase gene, just like BL21 (∆ispB::ddsA) used in this study. It is interesting that not only in these reconstructed bacteria, but in human-being, DPS can synthesize UQ-9 besides UQ-10. The mechanism of this phenomenon is still unknown. In this study, the effects of culture conditions, including the temperature, dissolved oxygen, pH and culture medium, on the DPS characteristics in BL21 (∆ispB::ddsA) were examined. Results show that temperature greatly affects the ratio of UQ-9/UQ-10, but not the total ubiquinone’s production. Increasing dissolved oxygen and protein concentration in culture medium can promote total ubiquinone’s production, but not the ratio of UQ-9/UQ-10. These results may give reference for UQ-10’s industrial produce and the mechanism of these conditions’ effect on DPS will be discussed.
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