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Culture Optimization for Enhanced Production of Microbial Pectin Methylesterase under Submerged Conditions

Author(s): S. Mandhania | V. Jain | S.P. Malhotra

Journal: Asian Journal of Biochemistry
ISSN 1815-9923

Volume: 5;
Issue: 1;
Start page: 12;
Date: 2010;
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Keywords: medium optimization | pectin methylesterase | Aspergillus heteromorphus

Aspergillus heteromorphus. The strain was added to its collection with identification number MTCC 9262. Various process variables viz., type and pH of the medium, temperature of growth, time of incubation, inoculum concentration and type and concentration of carbon and nitrogen sources were optimized as per the conventional method of one variable at a time approach which involves varying a single independent variable and maintaining others at a constant level. Modified pectin medium-A, in which both Fe++ and Cu++ were omitted, gave the maximum growth of A. heteromorphus. Maximum PME production was obtained when an inoculum size of 4x106 spores mL-1 was incubated in modified pectin medium-A for 144 h at pH 4.5 and at a temperature of 30C under static conditions in submerged fermentation. Out of the various nitrogen and carbon sources tested, dibasic ammonium phosphate and pectin were found to be the best N and C source, respectively. After standaradizing various process variables, a three fold increase in PME production by A. heteromorphus was achieved. Orange peel, a waste byproduct of fruit industry could effectively replace pectin as C-source thus, making the process cost effective. The PME produced by A. heteromorphus had the pH optimum in acidic range-a characteristic which could be exploited for its use in fruit processing industry.]]>
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