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Curing of Plasmid - Mediated Antibiotic Resistance in Multi-Drug Resistant Pathogens Using Alpinia galanga Rhizome Extract

Author(s): Varsha Shriram | Vinay Kumar | Javed Mulla | Latha. C

Journal: Advanced Biotech
ISSN 0973-0109

Volume: 13;
Issue: 01;
Start page: 01;
Date: 2013;
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Keywords: Antibiotic resistance | aqueous extract | methanol extract | multi-drug resistance | plasmid-curing

This study was in continuation of our earlier efforts in identifying phyto-derived agents as potential source for curing plasmid-mediated antibiotic resistance. Aqueous and methanol extracts of Alpinia galanga rhizomes were tried against multiple drug resistant (MDR) clinical isolates of Enterococcus faecalis, Staphylococcus aureus, Salmonella typhi, Shigella sonnei, as well as reference plasmid-harboring strains of Escherichia coli (RP4) and Bacillus subtilis (pUB110). Methanol extract exhibited bactericidal activities against reference plasmid harboring strains E. coli, B. subtilis and clinical isolate S. sonnei at 1200, 800 and 1200 µg/ml, respectively. Aqueous extract could not inhibit the growth of any pathogenic strain up to 1200 µg/ml concentrations. However, both the extracts were highly effective in curing plasmid-encoded antibiotic resistance in bacterial strains of clinical origin. Methanol extract showed plasmid curing at a much lower (400 µg/ml) concentration as compared to the aqueous extract (1200 µg/ml) and against wider range of clinical isolates. Methanol extracts showed a very high (98%) plasmid curing efficiency against S. typhi, followed by 50, 42 and 22 percent curing efficiencies against E. faecalis, S. aureus and S. sonnei respectively. Aqueous extract also showed noticeable antibiotic resistance reversal activities against R-plasmid harboring strains of clinical origin- E. faecalis, S. aureus and S. typhi with curing efficiencies of 72%, 70% and 12% espectively. The elimination of R-plasmids versed the multiple antibiotic esistances in cured derivatives making them sensitive to low concentrations of antibiotics. Both the extracts seem to be potential source for controlling the development and spread of plasmid-borne multiple antibiotic resistance
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