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Degradation Studies on Diesel Oil Using Bacterial Consortium Isolated from Oil Polluted Soil

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Author(s): Nisha. P | Nayana. M | Viji Varghese

Journal: Advanced Biotech
ISSN 0973-0109

Volume: 13;
Issue: 02;
Start page: 06;
Date: 2013;
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Keywords: Bacteria | Hydrocarbon | Diesel | Bacillus | Oil degradation .

ABSTRACT
Present study was conducted to evaluate the degradation efciency of bacterial consortium isolated from oil contaminated sites at Ernakulum District in India . Soil samples were used to isolate organisms. Isolated 5 pure cultures 2 Staphylococcus sp, Pseudomonas sp, 2 Bacillus sp were identied based on morphological, biochemical and physiological characters according to Bergey's Manual of determinative bacteriology. In-Vitro diesel degradation studies were done by using commercial diesel as a carbon source. In preliminary degradation studies,25ml of diesel was used in nutrient agar and but 25ml &12.5 ml were used in minimal agar to conrm the degradation capacity of test organisms. Oil recovery, width of oil layer and appearance were noted after 7 days of incubation. More potent strain E1 was allowed to grow in various parameters such as pH( 5,6,7,8 0 0),temperature ( 27 C,37 C), and diesel concentrations(12.5 ml ,6.25 ml, 3.12.5 ml ) assessed their pH change, nd th th st dry weight, optical density and enumeration of total viable bacterial count were determined in 2 , 7 , 15 & 21day.
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