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Detection of Paenibacillus larvae larvae spores in honey and diseased larvae Samples by culture and PCR

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Author(s): Moharrami, M., | Modirrousta, H., | Moeinfar, N.

Journal: Archives of Razi Institute
ISSN 0365-3439

Volume: 66;
Issue: 2;
Start page: 101;
Date: 2011;

Keywords: Hony bee | Paenibacillus Larvae | American Foulbrood(AFB) | Brood disease | PCR

ABSTRACT
American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods arereliable but rather slow simply because they are based on biochemical, morphological and physiologicalidentification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvaespores in honey and diseased larvae samples by culture and PCR. Therefore 54 samples of diseased larvaeand 36 honey samples, were diluted with an equal volume of distilled water and centrifuged, then the pelletwas used for bacterial culture, DNA extraction and PCR. PCR products were electrophoresed on 0.8 %agarose gel. Five of 54 (9.3 %) larvae samples and 5 of 36 (13.9 %) honey samples were positive forPaenibacillus larvae larvae by culture and PCR. As a result, screening of honey and larvae samples by PCRmethod proved to be a reliable, fast and useful method on regional and national scale for monitoring,controlling, and using preventive measures before the occurrence of American foulbrood damages.
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