Academic Journals Database
Disseminating quality controlled scientific knowledge

Determination of Key Intermediates in Cholesterol and Bile Acid Biosynthesis by Stable Isotope Dilution Mass Spectrometry

ADD TO MY LIST
 
Author(s): Tadashi Yoshida | Akira Honda | Hiroshi Miyazaki | Yasushi Matsuzaki

Journal: Analytical Chemistry Insights
ISSN 1177-3901

Volume: 3;
Start page: 45;
Date: 2008;
Original page

Keywords: isotope dilution mass spectrometry | biomarker | cholesterol synthesis | bile acid synthesis | mevalonate | oxysterol

ABSTRACT
For more than a decade, we have developed stable isotope dilution mass spectrometry methods to quantify key intermediates in cholesterol and bile acid biosynthesis, mevalonate and oxysterols, respectively. The methods are more sensitive and reproducible than conventional radioisotope (RI), gas-chromatography (GC) or high-performance liquid chromatography (HPLC) methods, so that they are applicable not only to samples from experimental animals but also to small amounts of human specimens. In this paper, we review the development of stable isotope dilution mass spectrometry for quantifying mevalonate and oxysterols in biological materials, and demonstrate the usefulness of this technique.
RPA Switzerland

RPA Switzerland

Robotic process automation

    

Tango Rapperswil
Tango Rapperswil