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Diagnóstico inmunológico de la Enfermedad de Chagas a partir de muestras colectadas en papel de filtro

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Journal: Salus Online
ISSN 1316-7138

Volume: 16;
Issue: 1;
Start page: 43;
Date: 2012;
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Keywords: Chagas Disease | diagnosis | filter paper

ABSTRACT
Chagas disease is produced by the parasite Trypanosoma cruzi. Immunological diagnosis methods are the most used, but they present problems of cross reactions with Leishmaniasis and angeliosis. For this reason, the World-Health-Organization (WHO) suggests the use of three different immunological tests. In Venezuela, the three tests are only available in research centers and laboratories of reference. Consequently, many samples must be transported under strict onditions of conservation, and despite this, they may become spoiled before arriving at the mentioned centers. The use of the filter paper could be an alternative for the collection and transportation of the samples. The aim of the present work was to compare the results of ELISA, HAI and IFI techniques using blood samples collected in tubes and in filter paper. The samples collected in tubes and filter paper were processed by ELISA, HAI and IFI techniques. For the filter paper samples elutions of 1/50 were done at various times for ELISA and IFI, and of 1/25 for HAI. Was obtained a sensibility of 91 % and specificity of 100 % in the final diagnosis of the samples collected in filter paper using the three techniques before mentioned, finding a kappa index of 0.91 (P
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