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Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections

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Author(s): Espeland Marianne | Irestedt Martin | Johanson Kjell | Åkerlund Monika | Bergh Jan-Erik | Källersjö Mari

Journal: Frontiers in Zoology
ISSN 1742-9994

Volume: 7;
Issue: 1;
Start page: 2;
Date: 2010;
Original page

ABSTRACT
Abstract Background The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, paradichlorobenzene and naphthalene on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time intervals. Results The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not. Conclusion Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should, however, be preferred due to physical damage of specimens and putative health risks by chemicals.
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