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Efeito de Isolados Bacterianos no Biocontrole ‘in vitro’ de Aspergillus sp. / Effect of the Isolated Bacteria in the Biocontrol ‘in vitro’ of Aspergillus sp.

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Author(s): S. VALIATI | E. FERRARI | H.F. SHIOMI

Journal: Scientific Electronic Archives
ISSN 2316-9281

Volume: 1;
Issue: 1;
Start page: 11;
Date: 2012;
Original page

Keywords: controle biológico | pós-colheita | micotoxinas | amendoim

ABSTRACT
RESUMO: Verificou-se em laboratório o efeito de sete isolados bacterianos sobre o crescimento micelial de Aspergillus sp. ‘in vitro’, a partir do método de “culturas pareadas”. O delineamento experimental utilizado foi o inteiramente casualizado, com oito tratamentos: T1 – Testemunha (Solução salina 0,85%), T2 – BB1 (Bacillus alcalophilus), T3 – BS3 (Photorhabdus luminescens), T4 – BB4 (Bacillus cereus GC. sub grupo B), T5 – BB5 (Stenotrophomonas maltophilia), T6 – BB6 (Yersinia bercovieri), T7 – BS5 (Bacillus cereus GC. sub grupo B) e T8 – BS6 (Bacillus cereus GC. sub grupo B) e três repetições cada. Avaliou-se o diâmetro do micélio do patógeno (cm) nos tratamentos com isolados quando a testemunha atingiu as extremidades da placa de Petri. Os dados coletados foram submetidos à análise de variância pelo teste F, através do software estatístico ESTAT e as diferenças entre médias foram comparadas pelo teste de Tukey, a 5% de probabilidade. Foi observado que os isolados bacterianos BB1, BB4, BB5, BS5 e BS6 não apresentaram nenhum efeito no controle do patógeno, igualando-se à testemunha. O tratamento com o isolado BS3 apresentou 7,8 cm de diâmetro, sendo considerado estatisticamente igual aos tratamentos sem efeito e ao melhor tratamento. No tratamento com o isolado BS6 o micélio do patógeno apresentou o menor valor, 6,03 cm, sendo estatisticamente superior aos demais.Unitermos: controle biológico, pós-colheita, micotoxinas, amendoim.ABSTRACT: It was found in the laboratory the effect of seven bacterial isolates on mycelial growth of Aspergillus sp. ‘in vitro’, by the method of "paired cultures". The experimental design was a completely randomized design with eight treatments: T1 - control (saline 0.85%), T2 - BB1 (B. alcalophilus), T3 - BS3 (P. luminescens), T4 - BB4 (B. cereus GC. subgroup B), T5 - BB5 (S. maltophilia), T6 - BB6 (Y. bercovieri), T7 - BS5 (B. cereus GC. subgroup B) and T8 - BS6 (B. cereus GC. subgroup B) and three repetitions each. Was evaluated the diameter of the pathogen mycelial (cm) in the isolates treatments when control has reached the ends of the Petri dish. The data collected were subjected to analysis of variance by F test, using the statistical software ESTAT and the differences between means were compared by Tukey test at 5% probability. It was observed that the bacterial isolates BB1, BB4, BB5, BS5 and BS6 had no effect on pathogen control, matching the witness. Treatment with isolated BS3 showed 7.8 cm in diameter, was considered statistically equal to ineffective treatments and the best treatment. In dealing with isolated BS6 the mycelium of the pathogen had the lowest value, 6.03 cm, which was statistically superior to the others.Keywords: biological control, post-harvest, mycotoxins, peanut.
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