Author(s): Gui-zhen XIAO | Fang-fang YUAN | Zheng-tao GU | Zhi-feng LIU | Ya-li ZHANG | Lei SU
Journal: Medical Journal of Chinese People's Liberation Army
ISSN 0577-7402
Volume: 38;
Issue: 6;
Start page: 472;
Date: 2013;
Original page
Keywords: heat stroke | intestinal mucosa | apoptosis
ABSTRACT
Objective To investigate the heat stress-induced dysfunction of intestinal barrier including intestinal tight junction and apoptosis of epithelial cells. Methods Human intestinal epithelial Caco-2 cell monolayers, serving as the intestinal barrier model, were exposed to different temperature (37-43℃) for designated time. Transepithelial electrical resistance (TEER) and horseradish peroxidase (HRP) flux permeability were measured to evaluate barrier integrity. Level of tight junction (TJ) protein occludin was analyzed by Western blotting. Cell apoptosis rate was determined using Annexin V-FITC/PI kit by flow cytometry. Results Compared with the 37℃ group, TEER lowered and the permeability for HRP increased significantly after heat exposure (P
Journal: Medical Journal of Chinese People's Liberation Army
ISSN 0577-7402
Volume: 38;
Issue: 6;
Start page: 472;
Date: 2013;
Original page
Keywords: heat stroke | intestinal mucosa | apoptosis
ABSTRACT
Objective To investigate the heat stress-induced dysfunction of intestinal barrier including intestinal tight junction and apoptosis of epithelial cells. Methods Human intestinal epithelial Caco-2 cell monolayers, serving as the intestinal barrier model, were exposed to different temperature (37-43℃) for designated time. Transepithelial electrical resistance (TEER) and horseradish peroxidase (HRP) flux permeability were measured to evaluate barrier integrity. Level of tight junction (TJ) protein occludin was analyzed by Western blotting. Cell apoptosis rate was determined using Annexin V-FITC/PI kit by flow cytometry. Results Compared with the 37℃ group, TEER lowered and the permeability for HRP increased significantly after heat exposure (P
