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Effectiveness of gelatin solidification for unfrozen preservation and transportation of astrocytes

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Author(s): Nobuko Ishibashi | Shinichi Sotome | Kazuyuki Nakajima | Akio Shimizu

Journal: Advances in Biological Chemistry
ISSN 2162-2183

Volume: 02;
Issue: 03;
Start page: 238;
Date: 2012;
Original page

Keywords: Cell preservation | Gelatin | Astrocytes | Unfrozen Preservation | Transportation

ABSTRACT
We herein studied a non-cryogenic cell preservation and transportation method. We found that astrocytes can be preserved in an unfrozen state at 4℃ (without medium exchange), while maintaining good condition in comparison with cryopreservation for the periods of 5 - 7 days. The gelatin solidification method can also prevent decreases in survival rate by preventing damage to astrocytes during transportation. Therefore, the gelatin solidification method at 4℃ may be a useful choice for short-term preservation and transportation.

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