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Effects of Dexamethasone on the proliferation and apoptosis of swine kidney fibroblast induced by TRAIL

Author(s): LI Xin | YANG Gong-She | JIA Qing | GUAN Wei-Jun | MA Yue-Hui

Journal: Acta Zoologica Sinica
ISSN 0001-7302

Volume: 54;
Issue: 5;
Start page: 836;
Date: 2008;
Original page

Keywords: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand(TRAIL) | Osteoprotegerin(OPG) | Dexamethasone | Fibroblast | Apoptosis

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraz-olium bromide (MTT) assay was applied to measure the cell growth. And flow cytometry (FCM) was adopted to detect the changes of FRSs cell cycle and apoptosis rate .In addition, the Semiquantitative RT-PCR was using to assessed the regulation of dexamethasone(DEX) for Osteoprotegerin(OPG) or Ligand of receptor activator of nuclear factor kappa B(RANKL) in FRS. The results showed that TRAIL could prompt the growth of swine kidney fibroblast at the concentrations of 0.01–5 mg/L, but TRAIL inhibited the growth of FRS at the concentrations of 10–50 mg/L, but at the concentrations of 10–50 mg/L, TRAIL inhibited the growth of swine kidney fibroblast and even led them to apoptosis. DEX could regulate the effects of cytokines. The effect of DEX on TRAIL is synergies and the effective concentrations of DEX are 10-6–10-10 mol/L. Cell cycle analysis and apoptosis detection showed that the peak concentration of TRAIL inducing FRSs’ proliferation was 5 mg/L. Under this concentration, TRAIL(5 mg/L)+DEX (10-8 mol/L) group showed that the proliferation index of FRSs (Prl.) was increased by 2.49% compared with TRAIL 5 mg/L group(P
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