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Effects of NAA BA and Sucrose On Shoot Induction And Rapid Micropropagation by Trimming Shoot Of Curcuma Longa L.

Author(s): Anchalee Jala

Journal: International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies
ISSN 2228-9860

Volume: 3;
Issue: 2;
Start page: 101;
Date: 2012;
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Keywords: Curcuma longa L. | BA | NAA | sucrose | trimming

Shoot tip of Curcuma longa L. were used as explants. These explants were cultured on MS medium supplemented with various concentration of (0, 1, 2, 3 and 4 mg/l ) BA and (0,0.5, and 1 mg/l) NAA. They were significant difference (p≤0.05) in each parameter. Explants which cultured on MS medium supplemented with 1 mg/l NAA and 2 or 3 mg/l BA gave the highest average number of new shoots ( 2.4, 2.6 shoots, respectively) and number of leaf (5.4 leaves), optimum number of roots ( 2.6 roots per shoot) and plant height (4.5 cm). In MS medium supplemented with only 2 mg/l BA produced the highest average number of shoot (2.6 shoots) and 5.4 leaves per shoot. When trimmed explants in longitudinal section (LS) to 2 and 3 sections. At 2 sections gave the highest number of new shoots (4.3 shoots per section). Explants which cultured on MS medium with 60 gm/l gave the highest average shoots and leaves per bunch, longest and biggest size of root.

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