Author(s): Jing-xi ZHANG | Li-jun ZHAO | Hui SHI | Chong BAI | Qiang LI
Journal: Medical Journal of Chinese People's Liberation Army
ISSN 0577-7402
Volume: 38;
Issue: 7;
Start page: 552;
Date: 2013;
Original page
Keywords: epithelial cells | poly I-C | lipopolysaccharides | peptidoglycan | immunity | natural | immunity | mucosal
ABSTRACT
Objective To study the effect of polyinosinic:polycytidylic acid (poly(I:C)), lipolysaccharide (LPS), peptidoglycan (PGN) on the barrier function and inflammatory mediators release of bronchial epithelium, and explore the impact of different specific Toll-like receptor (TLR 2, 3, 4) ligands on airway mucosal innate immune responses. Methods Polarized human bronchial epithelial cells (16HBEs) were cultured using transwell system to establish human bronchial epithelium model in vitro. The stimulation of Poly(I:C), LPS and PGN, respectively, was given on 16HBEs apically. The permeabilities of micromolecule and macromolecule between cells were measured by detecting trans-epithelial electrical resistance (TER) and basolateral medium FITC¬dextran concentrations after 24h stimulation. IL-8, GM-CSF and TNF-α protein content of apical and basal supernatants released by 16HBEs were measured by ELISA after 24h stimulation. Results 10μg/ml Poly(I:C) resulted in decrease of TER and reduction of increase of FITC-dextran permeability significantly (P
Journal: Medical Journal of Chinese People's Liberation Army
ISSN 0577-7402
Volume: 38;
Issue: 7;
Start page: 552;
Date: 2013;
Original page
Keywords: epithelial cells | poly I-C | lipopolysaccharides | peptidoglycan | immunity | natural | immunity | mucosal
ABSTRACT
Objective To study the effect of polyinosinic:polycytidylic acid (poly(I:C)), lipolysaccharide (LPS), peptidoglycan (PGN) on the barrier function and inflammatory mediators release of bronchial epithelium, and explore the impact of different specific Toll-like receptor (TLR 2, 3, 4) ligands on airway mucosal innate immune responses. Methods Polarized human bronchial epithelial cells (16HBEs) were cultured using transwell system to establish human bronchial epithelium model in vitro. The stimulation of Poly(I:C), LPS and PGN, respectively, was given on 16HBEs apically. The permeabilities of micromolecule and macromolecule between cells were measured by detecting trans-epithelial electrical resistance (TER) and basolateral medium FITC¬dextran concentrations after 24h stimulation. IL-8, GM-CSF and TNF-α protein content of apical and basal supernatants released by 16HBEs were measured by ELISA after 24h stimulation. Results 10μg/ml Poly(I:C) resulted in decrease of TER and reduction of increase of FITC-dextran permeability significantly (P
