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Electrophoretic profiles of gliadin subunits to evaluate genetic diversity of Triticum persicum Boiss. and Triticum pyramidale Percival

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Author(s): Ardashir Kharabian | Andy Jones | Behzad Kaviani

Journal: Plant Omics
ISSN 1836-0661

Volume: 1;
Issue: 1;
Start page: 9;
Date: 2008;
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Keywords: Alleles | A-PAGE | Gli-A1 | Marker | Variation | Wheat

ABSTRACT
The genetic diversity of two tetraploid wheat species were analyzed by examining gliadin seed storage protein markers at the Gli-A1 and Gli-B1 loci. Samples were collected from Iran, some areas of the Middle East and north of Africa (Egypt) comprising 15 populations of Triticum persicum and Triticum pyramidale. High rate of electrophoretic polymorphism was detected at the loci studied in two selected species. In T. persicum a total of 72 allelic variants at 2 loci were detected, including 41 for Gli-A1 and 31 for Gli-B1 . In the T. pyramidale samples, 50 allelic variants were totally observed. The number of alleles were 28 and 22 for Gli-A1 and Gli-B1 respectively. No null alleles were observed in the T. persicum samples. The total number of allelic variants in Iranian and othercountries samples of T.persicum were distinguished 42 and 30, respectively. In T.pyramidale conversely, the total number of allelic variants for Egyptian samples were 19 where is higher than other selected countries. In T.persicum population No 1 from Noorabad of Iran showed highest level of genetic diversity (A = 3.00, Ne = 2.11, He = 0.63 and GD = 0.51 ). In T.pyramidale population No,7 from cairo of Egypt had highest diversity ( A = 3.00, Ne = 2.50, He = 0.67 and GD = 0.57)
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