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Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads

Author(s): Xiaoying Guo | Yueping Guan | Bin Yang | Yongning Wang | Hualong Lan | Wentang Shi | Zhenghui Yang | Zuhong Lu

Journal: International Journal of Molecular Sciences
ISSN 1422-0067

Volume: 7;
Issue: 8;
Start page: 274;
Date: 2006;
Original page

Keywords: superparamagnetic polymer microbeads | modified suspension polymerization | enzyme chemiluminescence immunoassay | free hCGβ | tumor marker.

Superparamagnetic polymers (SPMP) microbead was an excellent form fornanosized magnetic particles less than 10nm to realize their potential applications in manyfields. We developed a novel modified suspension polymerization method for the productionof superparamagnetic poly (methacrylate divinylbenzene) (PMA-DVB) microbeads, withamino groups on their surface after simple modifications. We applied these SPMPmicrobeads to establish a sandwich enzyme chemiluminence immuno (ECLIA) procedure ofdetecting the free hCGβ in serum. It was proved to a better method compared to the ELISA,since it need half of the sample volume, simpler protocols, and the time it need wasshortened from 2 hours to 1 hour. The detection limit was 0.22 mIU•m-1, one order lowerthan the ELISA assay. Its linear range was between 0.45—185.2 mIU•m-1. And in thedetections of clinical serum samples, the related coefficient between the two methods’results was 0.955.
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